Journal article
Comparative Genomic Analyses of 17 Clinical Isolates of Gardnerella vaginalis Provide Evidence of Multiple Genetically Isolated Clades Consistent with Subspeciation into Genovars
Journal of bacteriology, v 194(15), pp 3922-3937
Aug 2012
PMID: 22609915
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Gardnerella vaginalis
is associated with a spectrum of clinical conditions, suggesting high degrees of genetic heterogeneity among stains. Seventeen
G. vaginalis
isolates were subjected to a battery of comparative genomic analyses to determine their level of relatedness. For each measure, the degree of difference among the
G. vaginalis
strains was the highest observed among 23 pathogenic bacterial species for which at least eight genomes are available. Genome sizes ranged from 1.491 to 1.716 Mb; GC contents ranged from 41.18% to 43.40%; and the core genome, consisting of only 746 genes, makes up only 51.6% of each strain's genome on average and accounts for only 27% of the species supragenome. Neighbor-grouping analyses, using both distributed gene possession data and core gene allelic data, each identified two major sets of strains, each of which is composed of two groups. Each of the four groups has its own characteristic genome size, GC ratio, and greatly expanded core gene content, making the genomic diversity of each group within the range for other bacterial species. To test whether these 4 groups corresponded to genetically isolated clades, we inferred the phylogeny of each distributed gene that was present in at least two strains and absent in at least two strains; this analysis identified frequent homologous recombination within groups but not between groups or sets.
G. vaginalis
appears to include four nonrecombining groups/clades of organisms with distinct gene pools and genomic properties, which may confer distinct ecological properties. Consequently, it may be appropriate to treat these four groups as separate species.
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Details
- Title
- Comparative Genomic Analyses of 17 Clinical Isolates of Gardnerella vaginalis Provide Evidence of Multiple Genetically Isolated Clades Consistent with Subspeciation into Genovars
- Creators
- Azad Ahmed - Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, Pennsylvania, USAJosh Earl - Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, Pennsylvania, USAAdam Retchless - Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, Pennsylvania, USASharon L Hillier - Magee-Womens Research Institute, Pittsburgh, Pennsylvania, USALorna K Rabe - Magee-Womens Research Institute, Pittsburgh, Pennsylvania, USAThomas L Cherpes - Magee-Womens Research Institute, Pittsburgh, Pennsylvania, USAEvan Powell - Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, Pennsylvania, USABenjamin Janto - Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, Pennsylvania, USARory Eutsey - Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, Pennsylvania, USAN. Luisa Hiller - Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, Pennsylvania, USARobert Boissy - Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, Pennsylvania, USAMargaret E Dahlgren - Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, Pennsylvania, USABarry G Hall - Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, Pennsylvania, USAJ. William Costerton - Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, Pennsylvania, USAJ. Christopher Post - Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, Pennsylvania, USAFen Z Hu - Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, Pennsylvania, USAGarth D Ehrlich - Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, Pennsylvania, USA
- Publication Details
- Journal of bacteriology, v 194(15), pp 3922-3937
- Publisher
- American Society for Microbiology; 1752 N St., N.W., Washington, DC
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000306634300017
- Scopus ID
- 2-s2.0-84866334429
- Other Identifier
- 991014877788404721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Microbiology