Journal article
Comparative ability of plasmid IL-12 and IL-15 to enhance cellular and humoral immune responses elicited by a SIVgag plasmid DNA vaccine and alter disease progression following SHIV 89.6P challenge in rhesus macaques
Vaccine, v 25(26), pp 4967-4982
2007
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Plasmid-based IL-12 has been demonstrated to successfully enhance the immunogenicity of DNA vaccines, thus enabling a reduction of the amount of DNA required for immunization. IL-15 is thought to affect the maintenance and enhance effector function of CD8
+ memory T cells. Since the ability to elicit a long-term memory response is a desirable attribute of a prophylactic vaccine, we sought to evaluate the ability of these plasmid-based cytokines to serve as vaccine adjuvants in rhesus macaques. Macaques were immunized with plasmid DNA encoding SIVgag in combination with plasmid IL-12, IL-15, or a combination of IL-12 and IL-15. The plasmid-based cytokines were monitored for their ability to augment SIVgag-specific cellular and humoral immune responses and to alter the clinical outcome following pathogenic SHIV
89.6P challenge. Macaques receiving SIVgag pDNA in combination with plasmid IL-12 alone, or in combination with plasmid IL-12 and IL-15, demonstrated significantly elevated cell-mediated and humoral immune responses resulting in an improved clinical outcome following virus challenge compared to macaques receiving SIVgag pDNA alone. Macaques receiving SIVgag pDNA in combination with plasmid IL-15 alone demonstrated minor increases in cell-mediated and humoral immune responses, however, the clinical outcome following virus challenge was not improved. These results have important implications for the continued development of plasmid DNA vaccines for the prevention of HIV-1 infection.
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Details
- Title
- Comparative ability of plasmid IL-12 and IL-15 to enhance cellular and humoral immune responses elicited by a SIVgag plasmid DNA vaccine and alter disease progression following SHIV 89.6P challenge in rhesus macaques
- Creators
- Siew-Yen Chong - Wyeth Vaccines Discovery, Pearl River, NY 10965, United StatesMichael A. Egan - Wyeth Vaccines Discovery, Pearl River, NY 10965, United StatesMichele A. Kutzler - University of PennsylvaniaShakuntala Megati - Human Immunome ProjectAmjed Masood - Human Immunome ProjectVidia Roopchard - Human Immunome ProjectDorys Garcia-Hand - Human Immunome ProjectDavid C. Montefiori - Duke University HospitalJorge Quiroz - Zimmer Biomet (United States)Margherita Rosati - National Cancer InstituteEva B. Schadeck - Human Immunome ProjectJean D. Boyer - University of PennsylvaniaGeorge N. Pavlakis - National Cancer InstituteDavid B. Weiner - University of PennsylvaniaManinder Sidhu - Human Immunome ProjectJohn H. Eldridge - Human Immunome ProjectZimra R. Israel - Human Immunome Project
- Publication Details
- Vaccine, v 25(26), pp 4967-4982
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Infectious Diseases (and HIV Medicine)
- Web of Science ID
- WOS:000247547400013
- Scopus ID
- 2-s2.0-34249689699
- Other Identifier
- 991020550495804721
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- Collaboration types
- Industry collaboration
- Domestic collaboration
- Web of Science research areas
- Immunology
- Medicine, Research & Experimental