Journal article
Comparing the Immune Response to PEEK as an Implant Material with and without P-15 Peptide as Bone Graft Material in a Rabbit Long Bone Model
Bioengineering (Basel), v 11(9), p898
06 Sep 2024
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
P-15 is a 15-amino-acid-long biomimetic peptide widely demonstrated to enhance osteogenesis in vivo. Despite the prevalence of polyether-ether-ketone (PEEK) in interbody device manufacturing, a growing body of evidence suggests it may produce an unfavorable immune response. The purpose of this preliminary study was to characterize the immune response and new bone growth surrounding PEEK implants with and without a P-15 peptide-based osteobiologic. A bilateral femoral defect model was conducted using New Zealand white rabbits. A total of 17 test subjects received one implant in each distal femur, either with or without bone graft material. Animals were allowed to survive to 4 or 8 weeks, at which time the femurs were collected and subjected to micro-computer tomography (microCT) or cytokine analysis. MicroCT analysis included the quantification of bone growth and density surrounding each implant. The cytokine analysis of periprosthetic tissue homogenates included the quantification of interleukins (ILs) and TNF-α expression via ELISA kits. Improvements in bone volume were observed in the P-15 cohort for the regions of interest, 500–136 and 136–0 µm from the implant surface, at 8 weeks post-op. Concentrations of IL-1β, IL-4, and IL-6 cytokines were significantly higher in the P-15 cohort compared to the PEEK cohort at the 4-week timepoint. Significant reductions in the concentrations of IL-4 and IL-6 cytokines from the 4- to 8-week cohort were observed in the P-15 cohort only. The P-15 peptide has the potential to modulate the immune response to implanted materials. We observed improvements in bone growth and a more active micro-environment in the P-15 cohort relative to the PEEK control. This may indicate an earlier transition from the inflammatory to remodeling phase of healing.
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Details
- Title
- Comparing the Immune Response to PEEK as an Implant Material with and without P-15 Peptide as Bone Graft Material in a Rabbit Long Bone Model
- Creators
- Boyle C. Cheng - Allegheny General HospitalIsaac R. Swink - Allegheny Health NetworkCooper T. Cheng - Allegheny General HospitalOwen G. Corcoran - Allegheny General HospitalVicki Z. Wang - Allegheny General HospitalEdward J. McClain McClain - Allegheny General HospitalPraveer S. Vyas - Allegheny General HospitalIzzy Owen - Allegheny General HospitalChen XuDaniel T. Altman - Allegheny General HospitalAlexander K. Yu - Allegheny General Hospital
- Publication Details
- Bioengineering (Basel), v 11(9), p898
- Publisher
- MDPI
- Number of pages
- 15
- Grant note
The authors declare that this study received funding from Cerapedics, Inc. The funder was not involved in the study design, collection, analysis, interpretation of data, the writing of this article or the decision to submit it for publication.
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Orthopedic/Orthopaedic Surgery; Surgery; Neurosurgery
- Web of Science ID
- WOS:001323060600001
- Scopus ID
- 2-s2.0-85205077431
- Other Identifier
- 991021903272004721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biotechnology & Applied Microbiology
- Engineering, Biomedical