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Comparison of Bacterial and Fungal Adherence to Vaginal Exfoliated Epithelial Cells and Human Vaginal Epithelial Tissue Culture Cells
Journal article   Open access   Peer reviewed

Comparison of Bacterial and Fungal Adherence to Vaginal Exfoliated Epithelial Cells and Human Vaginal Epithelial Tissue Culture Cells

M E Levison, J Sobel, P Myers and D Kaye
Infection and immunity, v 35(2), pp 697-701
01 Jan 1982
PMID: 6120141
url
https://doi.org/10.1128/iai.35.2.697-701.1982View
Published, Version of Record (VoR) Open

Abstract

adherence Candida albicans cell adhesion epithelium Escherichia coli Gardnerella vaginalis Lactobacillus Streptococcus group B vagina
The adherence of four bacterial species and Candida albicans to a new in vitro tissue culture model of human vaginal stratified squamous epithelium was investigated and compared with in vitro adherence to vaginal exfoliated cells. Gardnerella vaginalis , group B streptococci, Lactobacillus sp., and C. albicans adhered well to both exfoliated and tissue culture cells. Similarly, a piliated fecal isolate of Escherichia coli , but not a nonpiliated vaginal isolate of E. coli , adhered well to both cell types. Adherence of the piliated E. coli was markedly inhibited by preincubation of bacteria with D-mannose. No inhibition of adherence by D-mannose of G. vaginalis , nonpiliated E. coli , and C. albicans was demonstrated. Scanning electron microscopy of tissue cultures showed nonuniform distribution of adherent microorganisms with diminished adherence in areas of active mitosis and proliferation and increased adherence to mature flat cells, often in the process of desquamation.

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Collaboration types
Domestic collaboration
Web of Science research areas
Immunology
Infectious Diseases
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