Journal article
Comparison of hemerythrins from four species of sipunculids by optical absorption, circular dichroism, fluorescence emission, and resonance Raman spectroscopy
Biochemistry (Easton), Vol.16(8), pp.1743-1749
01 Jan 1977
PMID: 856259
Abstract
Resonance Raman, optical absorption, circular dichroic and fluorescence emission spectroscopy of hemerythrins from 4 spp. of sipunculids (Phascolopsis gouldii, Phascolosoma agassizii, Themiste dyscritum and T. pyroides) reveals no major differences in their active site or tertiary structures. This precludes any change in Fe ligands or coordination geometry and makes it unlikely that the active-site structures of P. gouldii and T. dyscritum hemerythrins could be as disparate as indicated by present crystallographic interpretations (Stenkamp et al 1976, Klotz, et al, 1976. Resonance Raman enhancement profiles of the stretching modes involving coordinated dioxygen maximize with excitation at .apprx. 525 nm, and correspond to the circular dichroic (CD) transition at .apprx. 520 nm. For coordinated azide modes in metazidohemerythrins these profiles maximize with excitation at .apprx. 505 nm corresponding to the 500-nm CD transition. Hemerythrins possess another resonance Raman peak at .apprx. 510 cm-1 which show maximum intensity enhancement at .apprx. 530 nm and this vibration is most likely associated with a permanent Fe- ligand.
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Details
- Title
- Comparison of hemerythrins from four species of sipunculids by optical absorption, circular dichroism, fluorescence emission, and resonance Raman spectroscopy
- Creators
- JBR Dunn - Portland State UniversityA W Addison - University of British ColumbiaR E Bruce - University of British ColumbiaJ S Loehr - Portland State UniversityT M Loehr - Portland State University
- Publication Details
- Biochemistry (Easton), Vol.16(8), pp.1743-1749
- Publisher
- Amer Chemical Soc
- Number of pages
- 7
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemistry
- Identifiers
- 991022027637604721