Logo image
Back
Comparison of oral fluid and serum ELISAs in the determination of IgG response to natural human papillomavirus infection in university women
Journal article   Peer reviewed

Comparison of oral fluid and serum ELISAs in the determination of IgG response to natural human papillomavirus infection in university women

Farrel J Buchinsky, Joseph J Carter, Gregory C Wipf, James P Hughes, Laura A Koutsky and Denise A Galloway
Journal of clinical virology, v 35(4), pp 450-453
01 Apr 2006
DOI: https://doi.org/10.1016/j.jcv.2005.09.014
PMID: 16316777
Featured in Collection :   UN Sustainable Development Goals @ Drexel

Abstract

Antibodies, Viral - blood Enzyme-Linked Immunosorbent Assay Female Human papillomavirus 16 - immunology Humans Immunoglobulin G - analysis Immunoglobulin G - blood Mouth Mucosa - immunology Papillomavirus Infections - diagnosis Papillomavirus Infections - immunology Papillomavirus Infections - virology Sensitivity and Specificity Students Universities Uterine Cervical Neoplasms - diagnosis Uterine Cervical Neoplasms - immunology Uterine Cervical Neoplasms - virology Washington
Venipuncture (phlebotomy) is an obstacle to subject recruitment and ongoing participation in cohort studies investigating human papillomavirus (HPV) infection. Anti-HPV antibodies are not only detected in serum but also in oral fluid. To evaluate if oral fluid specimens can be used in lieu of blood specimens for determining HPV antibody status. One hundred and seven paired oral fluid and blood specimens from female university students were tested in a HPV16 ELISA and compared to sexual history and serial genital HPV16 DNA status. ELISA results were in agreement in 97% (104/107) of paired sera and oral fluid. Of six women with positive anti-HPV16 serum samples, only three had positive oral fluid specimens. However, the specificity of the oral fluid test was 100% compared to the blood test. Detection of antibodies in oral fluid correlated with antibodies but was less sensitive than sera. A larger validation study is required to fully characterize the oral fluid assay.

Metrics

8 Record Views
13 citations in Web of Science
11 citations in Scopus

Details

UN Sustainable Development Goals (SDGs)

This publication has contributed to the advancement of the following goals:

#3 Good Health and Well-Being
#5 Gender Equality

InCites Highlights

Data related to this publication, from InCites Benchmarking & Analytics tool:

Collaboration types
Domestic collaboration
Web of Science research areas
Virology
Logo image