Journal article
Comparison of sea turtle thrombocyte aggregation to human platelet aggregation in whole blood
Comparative Biochemistry and Physiology, Part B, v 142(3), pp 353-360
2005
PMID: 16183311
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The endangered sea turtles are living “fossils” that afford us an opportunity to study the hemostatic process as it likely existed millions of years ago. There are essentially no data about turtle thrombocyte aggregation prior to our studies. Thrombocytes are nucleated cells that serve the same hemostatic functions as the anucleated mammalian platelet. Sea turtle thrombocytes aggregate in response to collagen and β-thrombin. Ristocetin induces an agglutination/aggregation response indicating the presence of a von Willebrand-like receptor, GPIb, found in all mammalian platelets. Samples treated with α-thrombin plus γ-thrombin followed by ristocetin results in a rapid, stronger response than ristocetin alone. These responses are inhibited by the RGDS peptide that blocks fibrinogen cross-linking of mammalian platelets via the fibrinogen receptor, GPIIb/IIIa. Three platelet-like proteins, GPIb, GPIIb/IIIa and P-selection are detected in sea turtle thrombocytes by fluorescence activated cell sorting. Turtle thrombocytes do not respond to ADP, epinephrine, serotonin, thromboxane A
2 mimetic, U46619, trypsin, or α-thrombin and γ-thrombin added alone. Comparison of hemostasis in sea turtles to other vertebrates could provide a framework for understanding the structure/function and evolution of these pathways and their individual components.
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Details
- Title
- Comparison of sea turtle thrombocyte aggregation to human platelet aggregation in whole blood
- Creators
- Gerald Soslau - Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, 245 N. 15th Street, MS 344, Philadelphia, PA 19102, USAPhillip J Prest - Medical Science Programs, Drexel University College of Medicine, Philadelphia, PA, USAReiner Class - Department Radiation Oncology, Drexel University College of Medicine, Philadelphia, PA, USARobert George - Virginia Aquarium and Marine Science Center, Gloucester Point, VA, USAFrank Paladino - Department of Biology, Purdue University, Fort Wayne, IN, USAGary Violetta - Sea World, Orlando, FL, USA
- Publication Details
- Comparative Biochemistry and Physiology, Part B, v 142(3), pp 353-360
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- [Retired Faculty]
- Web of Science ID
- WOS:000232967900012
- Scopus ID
- 2-s2.0-26844485158
- Other Identifier
- 991014877715704721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Zoology