Journal article
Comparison of tissue culture and animal models for assessment of Cryptospridium parvum infection
Experimental parasitology, v 101(2), pp 97-106
2002
PMID: 12427463
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The current increased interest for using tissue culture as a surrogate for mouse infection to assess
Cryptospridium viability suggests that a comparison of the two models is essential for data interpretation. Therefore, a need remains for a statistical comparison that can demonstrate if infection and inactivation predicted by new tissue culture models are comparable with those predicted by animal models. Data from a total of 31 dose–response trials using both tissue culture and mouse models to assess
C. parvum infectivity were compared. The dose needed to infect 50% of the tissue cultures (ID
50) was also compared to each ID
50 in mice. Average ID
50s developed using the logit dose–response method for tissue culture and mice were 8 and 107, respectively, suggesting that tissue culture was more sensitive to infection. However, correlation (
r) between tissue culture and mouse infectivity was statistically significant (0.9167 [95% CI=0.8428 to 0.9594,
p<0.0001]). Comparison of oocyst disinfection by UV and chlorine dioxide showed no significant difference between inactivation predicted by tissue culture and mouse models (
p=0.8893;
t=0.0141;
n=21). These results demonstrate that tissue culture can successfully be used to measure
C. parvum infection and can be used for determining inactivation in disinfection studies.
Metrics
Details
- Title
- Comparison of tissue culture and animal models for assessment of Cryptospridium parvum infection
- Creators
- Theresa R Slifko - Orange County Utilities Laboratory, Orlando, FL 32825, USADebra E Huffman - College of Marine Science, University of South Florida, 140 7th Ave. South, St. Petersburg, FL 33701, USABertrand Dussert - USFilter Wallace and Tiernan Products, 1901 West Garden Road, Vineland, NJ 08630, USAJames H Owens - USEPA, 26 West MLK Dr., Cincinnati, OH 45268, USAWalter Jakubowski - WaltJay Consulting, 6907 Maidmarian Court, Cincinnati, OH 45230-2222, USACharles N Haas - School of Environmental Science, Engineering, and Policy, Drexel University, 32nd and Chestnut Street Philadelphia, PA 19104, USAJoan B Rose - Michigan State University, Department of Crop and Soil Sciences, East Lansing, MI 48824-1325, USA
- Publication Details
- Experimental parasitology, v 101(2), pp 97-106
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Civil, Architectural, and Environmental Engineering
- Web of Science ID
- WOS:000179436600003
- Scopus ID
- 2-s2.0-0036628391
- Other Identifier
- 991014878465204721
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InCites Highlights
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Parasitology