Journal article
Compartmentalized DCC signalling is distinct from DCC localized to lipid rafts
Biology of the cell, v 101(2), pp 77-90
01 Feb 2009
PMID: 18616430
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Background information. Netrin-1 is a bi-functional cue that attracts or repels different classes of neurons during development. The netrin-1 receptor DCC (deleted in colorectal cancer) acts as a tyrosine kinase-associated receptor to mediate the attractive response towards netrin-1. The lipid raft-localized Src family kinase Fyn is required for DCC-mediated axon guidance. DCC functions are also dependent on lipid rafts, membrane microdomains corresponding to a low-density, detergent-resistant membrane fraction. However, it remains unclear how the association of DCC with lipid rafts controls netrin-1 signalling.
Results. DCC targeted to lipid rafts represented a minor proportion of total DCC inside the cell, but predominated on the cell surface of both IMR-32 human neuroblastoma cells and embryonic cortical neurons. Netrin-1 accumulated in lipid rafts, but had no effect on the targeting of DCC to that compartment, with DCC remaining on the cell surface in lipid rafts through 60 min post-treatment. However, DCC was able to interact with Fyn, both in the lipid rafts and soluble compartments isolated from embryonic El 9 rat brains, whereas early downstream signalling components such as Nck-1, and total and active focal adhesion kinase were mainly localized to the non-lipid raft compartment.
Conclusions. Together, these results suggest that DCC can be found in raft and non-raft portions of the plasma membrane, with early signalling events propagated by non-raft associated DCC.
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Details
- Title
- Compartmentalized DCC signalling is distinct from DCC localized to lipid rafts
- Creators
- Ryan J. Petrie - McGill UniversityBeibei Zhao - McGill UniversityFiona Bedford - McGill UniversityNathalie Lamarche-Vane - McGill University
- Publication Details
- Biology of the cell, v 101(2), pp 77-90
- Publisher
- Wiley
- Number of pages
- 14
- Grant note
- Fonds de la Recherche en Sante du Quebec (FRSQ); Fonds de la Recherche en Sante du Quebec CIHR-Canada Research Chair-Tier II; Canada Research Chairs; Canadian Institutes of Health Research (CIHR) MCIP147011 / Canadian Institute of Health Research (CIHR); Canadian Institutes of Health Research (CIHR) FRSQ Doctoral Studentship; Fonds de la Recherche en Sante du Quebec
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Web of Science ID
- WOS:000263283900002
- Scopus ID
- 2-s2.0-58149387268
- Other Identifier
- 991020100058804721
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- Cell Biology