Journal article
Complement fragment C3d is colocalized within the lipid rafts of T cells and promotes cytokine production
Lupus, v 21(12), pp 1294-1304
01 Oct 2012
PMID: 22851413
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The complement system plays an important role in tissue inflammation and damage in SLE patients. High levels of C3d were detected on the surface of erythrocytes and lymphocytes of SLE patients. The objective of this study was to assess the functional consequences of C3d fragments deposited on the surface membrane of SLE T cells. Methods: 46 SLE patients, 43 patients with other autoimmune diseases (OAD) and 33 healthy individuals (N) were enrolled in this study. T cells were isolated from peripheral blood and flow cytometry studies were conducted to assess the levels of C3d fragments, Ca++ influx responses and cytokine production. Confocal microscopy was used to study co-localized molecules. Student's t-test was performed to determine statistical significance among study groups. Results: A significant percentage of the SLE T cells were found to be positive for C3d (13.58 +/- 3.92%) when compared with normal T cells (4.52 +/- 2.92%) (p < 0.0000547) and T cells from patients with other autoimmune diseases (6.31 +/- 4.57%) (p < 0.00513). Peak Ca++ influx responses were significantly higher in C3d- SLE T cells compared with C3d+ SLE T cells (p < 0.011). C3d+ T cells produced significantly more IL-2, IFN-gamma, IL-4 and IL-17. In contrast to the increased production of IL-2 by the C3d+ T cells, the overall SLE T cell population produced less IL-2 when compared with T cells from normal individuals or patients with other autoimmune disease. The C3d fragments were found to be localized within the lipid rafts. Conclusion: C3d fragments are localized in the lipid rafts of SLE T cells and contribute to abnormal T cell function by modulating Ca++ influx responses and increased cytokine production. Lupus (2012) 21, 1294-1304.
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Details
- Title
- Complement fragment C3d is colocalized within the lipid rafts of T cells and promotes cytokine production
- Creators
- O. Borschukova - Beth Israel Deaconess Medical CenterZ. Paz - Beth Israel Deaconess Medical CenterI. C. Ghiran - Beth Israel Deaconess Medical CenterC-C Liu - University of PittsburghA. H. Kao - University of PittsburghS. Manzi - Allegheny Health NetworkJ. M. Ahearn - Allegheny-Singer Research InstituteG. C. Tsokos - Harvard University
- Publication Details
- Lupus, v 21(12), pp 1294-1304
- Publisher
- Sage
- Number of pages
- 11
- Grant note
- Lupus Research Institute R01HL096795 / NATIONAL HEART, LUNG, AND BLOOD INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Heart Lung & Blood Institute (NHLBI) W81XWH-06-2-0038 / Department of Defense; United States Department of Defense R01AI077591 / NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID) RO1AI077591; RO1HL0 96795 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Medicine (Graduate); General Internal Medicine
- Web of Science ID
- WOS:000309283100005
- Scopus ID
- 2-s2.0-84867004909
- Other Identifier
- 991021934011704721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Rheumatology