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Complex formation by the human Rad51B and Rad51C DNA repair proteins and their activities in vitro
Journal article   Open access   Peer reviewed

Complex formation by the human Rad51B and Rad51C DNA repair proteins and their activities in vitro

Yi-Ching Lio, Alexander V Mazin, Stephen C Kowalczykowski and David J Chen
The Journal of biological chemistry, v 278(4), pp 2469-2478
24 Jan 2003
DOI: https://doi.org/10.1074/jbc.M211038200
PMID: 12427746
Featured in Collection :   UN Sustainable Development Goals @ Drexel
url
https://doi.org/10.1074/jbc.M211038200View
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Abstract

Recombinant Proteins - metabolism Cell Line DNA, Single-Stranded - metabolism Electrophoresis, Polyacrylamide Gel Humans Insecta DNA - metabolism DNA-Binding Proteins - isolation & purification Magnetics DNA-Binding Proteins - chemistry DNA-Binding Proteins - metabolism Dose-Response Relationship, Drug Hydrolysis Sepharose - pharmacology Animals Time Factors Recombination, Genetic Adenosine Triphosphate - metabolism DNA Repair Protein Binding Protein Conformation Dimerization
The human Rad51 protein is essential for DNA repair by homologous recombination. In addition to Rad51 protein, five paralogs have been identified: Rad51B/Rad51L1, Rad51C/Rad51L2, Rad51D/Rad51L3, XRCC2, and XRCC3. To further characterize a subset of these proteins, recombinant Rad51, Rad51B-(His)(6), and Rad51C proteins were individually expressed employing the baculovirus system, and each was purified from Sf9 insect cells. Evidence from nickel-nitrilotriacetic acid pull-down experiments demonstrates a highly stable Rad51B.Rad51C heterodimer, which interacts weakly with Rad51. Rad51B and Rad51C proteins were found to bind single- and double-stranded DNA and to preferentially bind 3'-end-tailed double-stranded DNA. The ability to bind DNA was elevated with mixed Rad51 and Rad51C, as well as with mixed Rad51B and Rad51C, compared with that of the individual protein. In addition, both Rad51B and Rad51C exhibit DNA-stimulated ATPase activity. Rad51C displays an ATP-independent apparent DNA strand exchange activity, whereas Rad51B shows no such activity; this apparent strand exchange ability results actually from a duplex DNA destabilization capability of Rad51C. By analogy to the yeast Rad55 and Rad57, our results suggest that Rad51B and Rad51C function through interactions with the human Rad51 recombinase and play a crucial role in the homologous recombinational repair pathway.

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Collaboration types
Domestic collaboration
Web of Science research areas
Biochemistry & Molecular Biology
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