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Journal article
Comprehensive DNA methylation analysis of hepatitis B virus genome in infected liver tissues
Scientific reports, v 5(1), pp 10478-10478
22 May 2015
PMID: 26000761
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Hepatitis B virus (HBV) is a hepatotropic virus causing hepatitis, cirrhosis and hepatocellular carcinoma (HCC). The methylation status of the HBV DNA in its different forms can potentially provide insight into the pathogenesis of HBV-related liver diseases, including HCC, however this is unclear. The goal of this study is to obtain comprehensive DNA methylation profiles of the three putative CpG islands in the HBV DNA in infected livers, with respect to liver disease progression. The extent of methylation in these CpG islands was first assessed using bisulfite PCR sequencing with a small set of tissue samples, followed by analysis using both quantitative bisulfite-specific PCR and quantitative methylation-specific PCR assays in a larger sample size (n = 116). The level of HBV CpG island 3 methylation significantly correlated with hepatocarcinogenesis. We also obtained, for the first time, evidence of rare, non-CpG methylation in CpG island 2 of the HBV genome in infected liver. Comparing methylation of the HBV genome to three known HCC-associated host genes, APC, GSTP1, and RASSF1A, we did not identify a significant correlation between these two groups.
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Details
- Title
- Comprehensive DNA methylation analysis of hepatitis B virus genome in infected liver tissues
- Creators
- Surbhi Jain - JBS ScienceTing-Tsung Chang - National Cheng Kung UniversitySitong Chen - JBS ScienceBatbold Boldbaatar - JBS ScienceAdam Clemens - JBS ScienceSelena Y Lin - Drexel UniversityRan Yan - Drexel UniversityChi-Tan Hu - Tzu Chi UniversityHaitao Guo - Drexel UniversityTimothy M Block - Drexel UniversityWei Song - JBS Science (United States)Ying-Hsiu Su - Drexel University
- Publication Details
- Scientific reports, v 5(1), pp 10478-10478
- Publisher
- Springer Nature
- Grant note
- R01 AI094474 / NIAID NIH HHS R44 CA165312 / NCI NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology; A.J. Drexel Autism Institute
- Web of Science ID
- WOS:000355520600001
- Scopus ID
- 2-s2.0-84930227190
- Other Identifier
- 991019167569104721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Virology