Journal article
Concentration Dependence of a Hydrogel Phase Formed by the Deprotonation of the Imidazole Side Chain of Glycylhistidylglycine
Langmuir, v 37(23), pp 6935-6946
15 Jun 2021
PMID: 34077210
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Upon deprotonation of its imidazole group at similar to pH 6, the unblocked tripeptide glycylhistidylglycine (GHG) self-assembles into very long crystalline fibrils on a 10-1000 mu m scale which are capable of forming a volume spanning network, that is, hydrogel. The critical peptide concentration for self-assembly at a pH of 6 mM lies between 50 and 60 The fraction of peptides that self-assemble into fibrils depends on the concentration of deprotonated GHG. While IR spectra seem to indicate the formation of fibrils with standard amyloid fibril beta-sheet structures, vibrational circular dichroism spectra show a strongly enhanced amide I' signal, suggesting that the formed fibrils exhibit significant chirality. The fibril chirality appears to be a function of peptide concentration. Rheological measurements reveal that the rate of gelation is concentration-dependent and that there is an optimum gel strength at intermediate peptide concentrations of ca. 175 mM. This paper outlines the unique properties of the GHG gel phase which is underlain by a surprisingly dense fibril network with an exceptionally strong modulus that make them potential additives for biomedical applications.
Metrics
Details
- Title
- Concentration Dependence of a Hydrogel Phase Formed by the Deprotonation of the Imidazole Side Chain of Glycylhistidylglycine
- Creators
- Morgan Hesser - Drexel UniversityLavenia J. Thursch - Drexel UniversityTodd R. Lewis - Drexel UniversityThamires A. Lima - Drexel UniversityNicolas J. Alvarez - Drexel UniversityReinhard Schweitzer-Stenner - Drexel University
- Publication Details
- Langmuir, v 37(23), pp 6935-6946
- Publisher
- American Chemical Society; Washington, DC
- Number of pages
- 12
- Grant note
- Steinbright Career Development Center of Drexel University DMR170770; DMR-1915781 / National Science Foundation; National Science Foundation (NSF) Undergraduate research Co-op internship
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- [Retired Faculty]; Chemical and Biological Engineering
- Web of Science ID
- WOS:000663934200009
- Scopus ID
- 2-s2.0-85108302760
- Other Identifier
- 991019167434004721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Chemistry, Multidisciplinary
- Chemistry, Physical
- Materials Science, Multidisciplinary