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Journal article
Conserved 3′-Untranslated Region Sequences Direct Subcellular Localization of Chaperone Protein mRNAs in Neurons
The Journal of biological chemistry, v 285(23), pp 18025-18038
04 Jun 2010
PMID: 20308067
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
mRNA localization provides polarized cells with a locally renewable source of proteins. In neurons, mRNA translation can occur at millimeters to centimeters from the cell body, giving the dendritic and axonal processes a means to autonomously respond to their environment. Despite that hundreds of mRNAs have been detected in neuronal processes, there are no reliable means to predict mRNA localization elements. Here, we have asked what RNA elements are needed for localization of transcripts encoding endoplasmic reticulum chaperone proteins in neurons. The 3′-untranslated regions (UTRs) of calreticulin and Grp78/BiP mRNAs show no homology to one another, but each shows extensive regions of high sequence identity to their 3′UTRs in mammalian orthologs. These conserved regions are sufficient for subcellular localization of reporter mRNAs in neurons. The 3′UTR of calreticulin has two conserved regions, and either of these is sufficient for axonal and dendritic targeting. However, only nucleotides 1315–1412 show ligand responsiveness to neurotrophin 3 (NT3) and myelin-associated glycoprotein (MAG). This NT3- and MAG-dependent axonal mRNA transport requires activation of JNK, both for calreticulin mRNA and for other mRNAs whose axonal levels are commonly regulated by NT3 and MAG.
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Details
- Title
- Conserved 3′-Untranslated Region Sequences Direct Subcellular Localization of Chaperone Protein mRNAs in Neurons
- Creators
- Deepika Vuppalanchi - University of DelawareJennifer Coleman - Alfred I. duPont Hospital for ChildrenSoonmoon Yoo - Alfred I. duPont Hospital for ChildrenTanuja T. Merianda - Department of Biological Sciences, University of Delaware, Newark, Delaware 19716Akshay G. Yadhati - University of DelawareJobayer Hossain - University of DelawareArmin Blesch - University of California, San DiegoDianna E. Willis - Burke Medical Research InstituteJeffery L. Twiss - Drexel University
- Publication Details
- The Journal of biological chemistry, v 285(23), pp 18025-18038
- Publisher
- Elsevier
- Number of pages
- 14
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000278133400078
- Scopus ID
- 2-s2.0-77952923636
- Other Identifier
- 991021892107204721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology