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Journal article
Contact guidance is cell cycle-dependent
APL bioengineering, v 2(3), pp 31904-031904-13
01 Sep 2018
PMID: 29911682
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Cancer cell migration is essential for metastasis, during which cancer cells move through the tumor and reach the blood vessels. In vivo, cancer cells are exposed to contact guidance and chemotactic cues. Depending on the strength of such cues, cells will migrate in a random or directed manner. While similar cues may also stimulate cell proliferation, it is not clear whether cell cycle progression affects migration of cancer cells and whether this effect is different in random versus directed migration. In this study, we tested the effect of cell cycle progression on contact guided migration in 2D and 3D environments, in the breast carcinoma cell line, FUCCI-MDA-MB-231. The results were quantified from live cell microscopy images using the open source lineage editing and validation image analysis tools (LEVER). In 2D, cells were placed inside 10 mu m-wide microchannels to stimulate contact guidance, with or without an additional chemotactic gradient of the soluble epidermal growth factor. In 3D, contact guidance was modeled by aligned collagen fibers. In both 2D and 3D, contact guidance was cell cycle-dependent, while the addition of the chemoattractant gradient in 2D increased cell velocity and persistence in directionally migrating cells, regardless of their cell cycle phases. In both 2D and 3D contact guidance, cells in the G1 phase of the cell cycle outperformed cells in the S/G2 phase in terms of migration persistence and instantaneous velocity. These data suggest that in the presence of contact guidance cues in vivo, breast carcinoma cells in the G1 phase of the cell cycle may be more efficient in reaching the neighboring vasculature. (C) 2018 Author(s).
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Details
- Title
- Contact guidance is cell cycle-dependent
- Creators
- Kamyar Esmaeili Pourfarhangi - Temple UniversityEdgar Cardenas De la Hoz - Drexel UniversityAndrew R. Cohen - Drexel UniversityBojana Gligorijevic - Temple University
- Publication Details
- APL bioengineering, v 2(3), pp 31904-031904-13
- Publisher
- American Institute of Physics
- Number of pages
- 13
- Grant note
- 5K99CA172360 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA Concern Foundation R01NS076709 / NIH NINDS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS) R01AG041861 / NATIONAL INSTITUTE ON AGING; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute on Aging (NIA) R00CA172360 / NATIONAL CANCER INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI) R01AG041861 / NIH NIA; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute on Aging (NIA)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Electrical and Computer Engineering
- Web of Science ID
- WOS:000455060700008
- Scopus ID
- 2-s2.0-85055501343
- Other Identifier
- 991019168393604721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Engineering, Biomedical