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Journal article
Contractile Smooth Muscle and Active Stress Generation in Porcine Common Carotids
Journal of biomechanical engineering, v 140(1), pp 0145011-0145016
01 Jan 2018
PMID: 28975258
Abstract
The mechanical response of intact blood vessels to applied loads can be delineated into passive and active components using an isometric decomposition approach. Whereas the passive response is due predominantly to the extracellular matrix (ECM) proteins and amorphous ground substance, the active response depends on the presence of smooth muscle cells (SMCs) and the contractile machinery activated within those cells. To better understand determinants of active stress generation within the vascular wall, we subjected porcine common carotid arteries (CCAs) to biaxial inflation-extension testing under maximally contracted or passive SMC conditions and semiquantitatively measured two known markers of the contractile SMC phenotype: smoothelin and smooth muscle-myosin heavy chain (SM-MHC). Using isometric decomposition and established constitutive models, an intuitive but novel correlation between the magnitude of active stress generation and the relative abundance of smoothelin and SM-MHC emerged. Our results reiterate the importance of stretch-dependent active stress generation to the total mechanical response. Overall these findings can be used to decouple the mechanical contribution of SMCs from the ECM and is therefore a powerful tool in the analysis of disease states and potential therapies where both constituent are altered.
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Details
- Title
- Contractile Smooth Muscle and Active Stress Generation in Porcine Common Carotids
- Creators
- Boran Zhou - Mayo ClinicDavid A Prim - University of South CarolinaEva J Romito - University of South CarolinaLiam P McNamara - University of South CarolinaFrancis G Spinale - University of South CarolinaTarek Shazly - University of South CarolinaJohn F Eberth - University of South Carolina
- Publication Details
- Journal of biomechanical engineering, v 140(1), pp 0145011-0145016
- Publisher
- ASME
- Grant note
- R01 HL130972 / NHLBI NIH HHS R21 EB022131 / NIBIB NIH HHS P20 GM103499 / NIGMS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000415721600012
- Scopus ID
- 2-s2.0-85034041156
- Other Identifier
- 991021902501004721
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biophysics
- Engineering, Biomedical