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Contrasting methods to operationalize antibiotic exposure in clinical research: a real-world application on health care-associated Clostridioides difficile infection
Journal article   Open access   Peer reviewed

Contrasting methods to operationalize antibiotic exposure in clinical research: a real-world application on health care-associated Clostridioides difficile infection

Jessica L. Webster, Stephen Eppes, Brian K. Lee, Nicole S. Harrington and Neal D. Goldstein
American journal of epidemiology, v 194(5), pp 1448-1459
07 May 2025
PMID: 39191653
url
https://doi.org/10.1093/aje/kwae302View
Published, Version of Record (VoR) Open

Abstract

Life Sciences & Biomedicine Public, Environmental & Occupational Health Science & Technology
The goal of this article is to summarize common methods of antibiotic operationalization used in clinical research and demonstrate methods for exposure variable selection. We demonstrate 3 methods for modeling exposure, using data from a case-control study on Clostridioides difficile infection in hospitalized patients: (1) factor analysis, (2) logistic regression models, and 3) least absolute shrinkage and selection operator (LASSO) regression. The factor analysis identified 8 variables contributing the most variation in the data set: any antibiotic exposure; number of antibiotic classes; number of antibiotic courses; dose; and specific classes monobactam, beta-lactam-beta-lactamase inhibitors, rifamycin, and cephalosporin. The logistic regression models resulting in the best model fit used predictors representing any antibiotic exposure and the proportion of a patient's hospitalization that they were receiving antibiotics. The LASSO model selected 22 variables for inclusion in the predictive model, of which 10 were antibiotic exposure variables, including any antibiotic exposure; classes beta-lactam-beta-lactamase inhibitors, carbapenem, cephalosporin, fluoroquinolone, monobactam, rifamycin, sulfonamides, and miscellaneous; and proportion of hospitalization that antibiotic treatment was administered. Investigators studying antibiotic use should consider multiple characteristics of exposure informed by their research question and the theory on how antibiotics may affect the distribution of the outcome in their target population.

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Public, Environmental & Occupational Health
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