Journal article
Control of mitochondrial matrix calcium: Studies using fluo-3 as a fluorescent calcium indicator
Biochemical and biophysical research communications, v 167(1)
1990
PMID: 2310386
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Abstract
Fluo-3, a fluorescent Ca
2+ indicator, is sequestered by isolated rat liver mitochondria and is an effective probe for evaluating the concentration and kinetics of change of mitochondrial matrix ionized calcium ([Ca
2+]m) under a variety of conditions. At the wavelengths employed, there is no significant interference by auto-fluorescence. There is an insignificant release of the indicator over four hours and the loading and presence of fluo-3 has no effect on respiratory rate or oxidative phosphorylation. The [Ca
2+]m steady state can be altered by the assay conditions, i.e. the presence of extramitochondrial Ca
2+, Mg
2+, phosphate and respiratory inhibitors. The total matrix ionized calcium represents a small percent (<0.01%) of the total mitochondrial calcium.
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Details
- Title
- Control of mitochondrial matrix calcium: Studies using fluo-3 as a fluorescent calcium indicator
- Creators
- Alfredo Saavedra-Molina - Hahnemann University HospitalSalvador Uribe - National Autonomous University of MexicoThomas M. Devlin - Hahnemann University Hospital
- Publication Details
- Biochemical and biophysical research communications, v 167(1)
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:A1990CQ50300023
- Scopus ID
- 2-s2.0-0025234537
- Other Identifier
- 991019183923104721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Biophysics