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Controlled release of anti-inflammatory agent alpha-MSH from neural implants
Journal article   Peer reviewed

Controlled release of anti-inflammatory agent alpha-MSH from neural implants

Yinghui Zhong and Ravi V Bellamkonda
Journal of controlled release, v 106(3)
02 Sep 2005
PMID: 15978692

Abstract

Microglia - metabolism Nitric Oxide - biosynthesis alpha-MSH - chemistry Microglia - drug effects Drug Implants Rats Rats, Sprague-Dawley Brain - metabolism Electrodes Animals Anti-Inflammatory Agents - administration & dosage Collodion - administration & dosage alpha-MSH - administration & dosage
Si-multi-electrode arrays implanted into brain tissue for long-term recording lose electrical connectivity due to the post-implantation inflammatory reaction. This inflammatory reaction creates a physical and electrical gap between the electrode and the surrounding neurons. In this study, novel nitrocellulose-based coatings were developed for the sustained delivery of the anti-inflammatory neuropeptide alpha-melanocyte stimulating hormone (alpha-MSH). alpha-MSH was incorporated in micron-scale nitrocellulose coatings and slow, sustained release over 21 days was attained in vitro. The alpha-MSH released on day 21 was still bioactive, and successfully inhibited nitric oxide (NO) production by LPS-stimulated microglia. The amount of initial drug loading directly affected the release rate, with higher initial loading increasing the mass released but not the percent of drug released. The surface morphology and thickness of the coatings were examined by scanning electron microscopy (SEM) and profilometry. In addition, impedance measurement showed that the alpha-MSH loaded nitrocellulose coatings reduced the magnitude of electrode impedance at the biologically relevant frequency of 1 kHz. In conclusion, nitrocellulose-based, bioactive coatings that release anti-inflammatory agents without increasing the impedence of the electrode were successfully fabricated. These coatings have the potential to reduce inflammation at the electrode-brain interface in vivo, and facilitate long-term recordings from Si-multi-electrode arrays.

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Web of Science research areas
Chemistry, Multidisciplinary
Pharmacology & Pharmacy
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