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Correlation between Presence of Viable Bacteria and Presence of Endotoxin in Middle-Ear Effusions
Journal article   Open access   Peer reviewed

Correlation between Presence of Viable Bacteria and Presence of Endotoxin in Middle-Ear Effusions

Jeffrey R Dingman, Mark G Rayner, Suman Mishra, Yingze Zhang, Miles D Ehrlich, J. Christopher Post and Garth D Ehrlich
Journal of clinical microbiology, v 36(11), pp 3417-3419
Nov 1998
PMID: 9774611
url
https://doi.org/10.1128/JCM.36.11.3417-3419.1998View
Published, Version of Record (VoR) Open

Abstract

Bacteriology
The presence of endotoxin (detected by the Limulus amebocyte lysate assay) was compared to the presence of viable Haemophilus influenzae and Moraxella catarrhalis (detected by PCR) in 106 middle-ear effusions from pediatric patients with chronic otitis media. Endotoxin was found in 81 of the 106 specimens. Of these 81 specimens, 66 (81.5%) also tested positive for one or both of the gram-negative bacteria H. influenzae and M. catarrhalis . The data suggest that viable gram-negative bacteria, detectable by PCR but often undetectable by culture, may be the source of endotoxin in middle-ear effusions.

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Web of Science research areas
Microbiology
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