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Correlation of C3b-receptor activity and diphenylhexatriene polarization in a murine macrophage cell line
Journal article   Peer reviewed

Correlation of C3b-receptor activity and diphenylhexatriene polarization in a murine macrophage cell line

M. Esfahani, P.H. Saluk, D.R. Tabor, L. Scerbo and T.M. Devlin
Biochemical and biophysical research communications, v 109(2), pp 328-333
1982
PMID: 6217816

Abstract

Treatment of a macrophage cell line with lymphokine (LK)-rich lymphocyte culture supernatants activates the cells for C3b receptor-mediated phagocytosis and leads to simultaneous depolarization of 1,6-diphenyl-1,3,5-hexatriene (DPH) incorporated into the cells. Maximal effects were observed after 14 hours of incubation with LK. DPH polarization in liposomes prepared from lipids of untreated and LK-treated cells were the same. Furthermore, LK-treatment had no significant effects on phospholipid fatty acid composition, cholesterol content, or phospholipid composition. These observations indicate LK induces alterations in the organization and dynamics of the plasma membrane that lead to facilitation of phagocytosis.

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Web of Science research areas
Biochemistry & Molecular Biology
Biophysics
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