Journal article
Correlative light and backscattered electron microscopy of bone —Part II: Automated image analysis
Scanning, v 22(6), pp 337-344
Nov 2000
PMID: 11145258
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Detailed studies of biological phenomena often involve multiple microscopy and imaging modes and media. For bone biology, various forms of light and electron microscopy are used to study the microscopic structure of bone. Integrating information from the different sources is necessary to understand how different aspects of the bone structure interact. To accomplish this, methods were developed to prepare and image thin sections for cor‐relative light microscopy (LM) and backscattered electron imaging in the scanning electron microscope (BSE‐SEM). Images of the same fields of view may then be analyzed for degrees of relationships between specimen features not observed by LM or SEM alone. These methods are applied here to study possible associations between the degree of bone mineralization and pattern of collagen fiber orientation in the mid‐shaft of the human femur. The “relational images” obtained allow us to examine the relationship between these two variables, both objectively and quantitatively.
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Details
- Title
- Correlative light and backscattered electron microscopy of bone —Part II: Automated image analysis
- Creators
- Haviva M GoldmanAron BlayvasAlan BoydePeter G. T HowellJohn G ClementTimothy G Bromage
- Publication Details
- Scanning, v 22(6), pp 337-344
- Publisher
- Wiley Periodicals, Inc; New Jersey
- Number of pages
- 8
- Grant note
- The Louis B. Leakey Foundation National Science Foundation (NSF) National Aeronautical and Space Administration (NASA) Horserace Betting Levy Board
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000165779100001
- Scopus ID
- 2-s2.0-0034487998
- Other Identifier
- 991014878091904721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Instruments & Instrumentation
- Microscopy