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Correlative light and backscattered electron microscopy of bone—Part I: Specimen preparation methods
Journal article   Open access   Peer reviewed

Correlative light and backscattered electron microscopy of bone—Part I: Specimen preparation methods

Haviva M Goldman, John Kindsvater and Timothy G Bromage
Scanning, v 21(1), pp 40-43
Jan 1999
PMID: 10070782
url
https://doi.org/10.1002/sca.4950210106View
Published, Version of Record (VoR)Maybe Open Access (Publisher Bronze) Open

Abstract

scanning electron microscopy backscattered electron microscopy correlative studies light cure dental adhesives light microscopy
A method for preparing nondecalcified bone and tooth specimens for imaging by both light microscopy (LM) and backscattered electron microscopy in the scanning electron microscope (B SE‐SEM) is presented. Bone blocks are embedded in a polymethylmethacrylate (PMMA) mixture and mounted on glass slides using components of a light‐cured dental adhesive system. This method of slide preparation allows correlative studies to be carried out between different microscopy modes, using the same histologic section. It also represents a large time savings relative to other mounting methods whose media require long cure times.

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Web of Science research areas
Instruments & Instrumentation
Microscopy
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