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Covalently incorporated protein-nanogels using AGET ATRP in an inverse miniemulsion
Journal article   Peer reviewed

Covalently incorporated protein-nanogels using AGET ATRP in an inverse miniemulsion

Saadyah E. Averick, Andrew J. D. Magenau, Antonina Simakova, Bradley F. Woodman, Andrew Seong, Ryan A. Mehl and Krzysztof Matyjaszewski
Polymer chemistry, v 2(7), pp 1476-1478
01 Jan 2011
DOI: https://doi.org/10.1039/c1py00050k

Abstract

Physical Sciences Polymer Science Science & Technology
Using a genetically engineered protein, containing a non-natural amino acid bearing an atom transfer radical polymerization (ATRP) initiator, protein-nanogel hybrids (PNHs) were synthesized by activator generated by electron transfer (AGET) ATRP in an inverse miniemulsion. The route presented is an appropriate synthetic strategy to covalently and site specifically incorporate green fluorescent protein (GFP) into well-defined nanogels. These PNHs were analyzed using dynamic light scattering (DLS), UV-visible fluorescence spectroscopy and confocal microscopy to confirm the successful integration of GFP proteins into each nanogel (NG), while preserving its native tertiary structure.

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Domestic collaboration
Web of Science research areas
Polymer Science
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