Journal article
Cu(II) and Ni(II) Interactions with the Terminally Blocked Hexapeptide Ac-Leu-Ala-His-Tyr-Asn-Lys-amide Model of Histone H2B (80–85)
Bioinorganic chemistry and applications, v 2008, pp 1-10
2008
PMID: 18431450
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The N- and C-terminal blocked hexapeptide Ac-Leu-Ala-His-Tyr-Asn-Lys-amide (LAHYNK) representing the 80–85 fragment of histone H2B was synthesized and its interactions with Cu(II) and Ni(II) ions were studied by potentiometric, UV-Vis, CD, EPR, and NMR spectroscopic techniques in solution. Our data reveal that the imidazole N(3) nitrogen atom is the primary ligating group for both metal ions. Sequential amide groups deprotonation and subsequent coordination to metal ions indicated an coordination mode above , in all cases. In the case of Cu(II)-peptide system, the almost exclusive formation of the predominant species CuL in neutral media accounting for almost 98% of the total metal ion concentration at pH 7.3 strongly indicates that at physiological pH values the sequence -LAHYNK- of histone H2B provides very efficient binding sites for metal ions. The imidazole pyrrole N(1) ionization (but not coordination) was also detected in species CuH-4L present in solution above .
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Details
- Title
- Cu(II) and Ni(II) Interactions with the Terminally Blocked Hexapeptide Ac-Leu-Ala-His-Tyr-Asn-Lys-amide Model of Histone H2B (80–85)
- Creators
- Katerina Panagiotou - Department of Chemistry, University of Ioannina, 45110 Ioannina, GreeceMaria Panagopoulou - Department of Chemistry, University of Ioannina, 45110 Ioannina, GreeceTilemachos Karavelas - Department of Chemistry, University of Ioannina, 45110 Ioannina, GreeceVassiliki Dokorou - Department of Chemistry, University of Ioannina, 45110 Ioannina, GreeceAndrew Hagarman - Department of Chemistry, Drexel University, 3141 Chestnut Street, Philadelphia, PA 19104, USAJonathan Soffer - Department of Chemistry, Drexel University, 3141 Chestnut Street, Philadelphia, PA 19104, USAReinhard Schweitzer-Stenner - Department of Chemistry, Drexel University, 3141 Chestnut Street, Philadelphia, PA 19104, USAGerasimos Malandrinos - Department of Chemistry, University of Ioannina, 45110 Ioannina, GreeceNick Hadjiliadis - Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece
- Publication Details
- Bioinorganic chemistry and applications, v 2008, pp 1-10
- Publisher
- Wiley
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemistry
- Web of Science ID
- WOS:000255639900001
- Scopus ID
- 2-s2.0-43949119411
- Other Identifier
- 991014877959004721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Chemistry, Inorganic & Nuclear
- Chemistry, Organic