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Cyclin D1 induction of Dicer governs microRNA processing and expression in breast cancer
Journal article   Open access   Peer reviewed

Cyclin D1 induction of Dicer governs microRNA processing and expression in breast cancer

Zuoren Yu, Liping Wang, Chenguang Wang, Xiaoming Ju, Min Wang, Ke Chen, Emanuele Loro, Zhiping Li, Yuzhen Zhang, Kongming Wu, …
Nature communications, v 4(1), pp 2812-2812
01 Nov 2013
PMID: 24287487
url
https://doi.org/10.1038/ncomms3812View
Published, Version of Record (VoR)Maybe Open Access (Publisher Bronze) Open

Abstract

Multidisciplinary Sciences Science & Technology Science & Technology - Other Topics
Cyclin D1 encodes the regulatory subunit of a holoenzyme that phosphorylates the pRB protein and promotes G(1)/S cell-cycle progression and oncogenesis. Dicer is a central regulator of miRNA maturation, encoding an enzyme that cleaves double-stranded RNA or stem-loop-stem RNA into 20-25 nucleotide long small RNA, governing sequence-specific gene silencing and heterochromatin methylation. The mechanism by which the cell cycle directly controls the non-coding genome is poorly understood. Here we show that cyclin D1(-/-) cells are defective in pre-miRNA processing which is restored by cyclin D1a rescue. Cyclin D1 induces Dicer expression in vitro and in vivo. Dicer is transcriptionally targeted by cyclin D1, via a cdk-independent mechanism. Cyclin D1 and Dicer expression significantly correlates in luminal A and basal-like subtypes of human breast cancer. Cyclin D1 and Dicer maintain heterochromatic histone modification (Tri-m-H3K9). Cyclin D1-mediated cellular proliferation and migration is Dicer-dependent. We conclude that cyclin D1 induction of Dicer coordinates microRNA biogenesis.

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Cell Biology
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