Journal article
Cytokine Regulation of the Matrix Metalloproteinases and Their Inhibitors in Human Papillomavirus-18 Transformed Human Prostatic Tumor Cell Lines
Oncology research, v 8(7-8), pp 303-315
01 Jan 1996
PMID: 8938794
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Abstract Cytokines may play a critical role in influencing the invasive and metastatic behavior of advanced cancers. To investigate the influence of cytokines on tissue inhibitor of metalloproteinase (TIMP) and matrix metalloproteinase (MMP) expression we have established
cultures from prostate tissues of low Gleason sum 5 and high Gleason sum 10 cancers. We have examined the influence of different cytokines (interleukin [IL]-10, IL-4, IL-6, IL-2, and interferon-γ) on TIMP-1, TIMP-2, MMP-2, and MMP-9 protein and mRNA expression in human papillomavirus
(HPV)-18 immortalized human prostate cell lines derived from the primary cultures. Western blot and northern blot analysis revealed that IL-10, IL-6 and IL-4 all upregulated TIMP-1 expression after 16-36 h. In contrast, IL-10 and IL-4 (but not IL-6) downregulated MMP-2 mRNA and protein levels
to different degrees over 24-36 h. The levels of TTMP-2 and MMP-9 protein and mRNA were not influenced substantially by any of the cytokines. Also, IL-2 and interferon-γ had little or no effect on any of these genes. In sum, the data showed that IL-10 (or IL-4) upregulated TIMP-1 and
coordinately downregulated MMP-2 expression. Thus, cytokines might control the molar ratio of TIMP-1 and MMP-2 to influence the level of protease activity and perhaps the invasive behavior of malignant cells in vivo.
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Details
- Title
- Cytokine Regulation of the Matrix Metalloproteinases and Their Inhibitors in Human Papillomavirus-18 Transformed Human Prostatic Tumor Cell Lines
- Creators
- Min WangKim FudgeJahn S RhimMark E Stearns
- Publication Details
- Oncology research, v 8(7-8), pp 303-315
- Publisher
- Cognizant Communication Corporation
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Decision Sciences (and Management Information Systems)
- Web of Science ID
- WOS:A1996VU94800006
- Scopus ID
- 2-s2.0-0030463727
- Other Identifier
- 991019167823604721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Oncology