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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Cytoplasmic pressure maintains epithelial integrity and inhibits cell motility
Physical biology, v 18(6), p66003
04 Oct 2021
PMID: 34521072
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Cytoplasmic pressure, a function of actomyosin contractility and water flow, can regulate cellular morphology and dynamics. In mesenchymal cells, cytoplasmic pressure powers cell protrusion through physiological three-dimensional extracellular matrices. However, the role of intracellular pressure in epithelial cells is relatively unclear. Here we find that high cytoplasmic pressure is necessary to maintain barrier function, one of the hallmarks of epithelial homeostasis. Further, our data show that decreased cytoplasmic pressure facilitates lamellipodia formation during the epithelial to mesenchymal transition (EMT). Critically, activation of the actin nucleating protein Arp2/3 is required for the reduction in cytoplasmic pressure and lamellipodia formation in response to treatment with hepatocyte growth factor (HGF) to induce EMT. Thus, elevated cytoplasmic pressure functions to maintain epithelial tissue integrity, while reduced cytoplasmic pressure triggers lamellipodia formation and motility during HGF-dependent EMT.
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Details
- Title
- Cytoplasmic pressure maintains epithelial integrity and inhibits cell motility
- Creators
- Tia M Jones - Drexel UniversityPragati C Marks - Drexel UniversityJames M Cowan - Drexel UniversityDevneet K Kainth - Drexel UniversityRyan J Petrie - Drexel University
- Publication Details
- Physical biology, v 18(6), p66003
- Publisher
- Institute of Physics (IOP)
- Grant note
- R01 GM126054 / NIGMS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology; College of Medicine
- Web of Science ID
- WOS:000712045300001
- Scopus ID
- 2-s2.0-85119477278
- Other Identifier
- 991019167735504721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology
- Biophysics