Journal article
Cytotoxic macrophage-released tumour necrosis factor-alpha (TNF-alpha) as a killing mechanism for cancer cell death after cold plasma activation
Journal of physics. D, Applied physics, v 49(8), pp 84001-84009
02 Mar 2016
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The present study aims at studying the anticancer role of cold plasma-activated immune cells. The direct anti-cancer activity of plasma-activated immune cells against human solid cancers has not been described so far. Hence, we assessed the effect of plasma-treated RAW264.7 macrophages on cancer cell growth after co-culture. In particular, flow cytometer analysis revealed that plasma did not induce any cell death in RAW264.7 macrophages. Interestingly, immunofluorescence and western blot analysis confirmed that TNF-alpha released from plasma-activated macrophages acts as a tumour cell death inducer. In support of these findings, activated macrophages down-regulated the cell growth in solid cancer cell lines and induced cell death in vitro. Together our findings suggest plasma-induced reactive species recruit cytotoxic macrophages to release TNF-alpha, which blocks cancer cell growth and can have the potential to contribute to reducing tumour growth in vivo in the near future.
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Details
- Title
- Cytotoxic macrophage-released tumour necrosis factor-alpha (TNF-alpha) as a killing mechanism for cancer cell death after cold plasma activation
- Creators
- Nagendra Kumar Kaushik - Kwangwoon UniversityNeha Kaushik - Kwangwoon UniversityBooki Min - Kwangwoon Univ, Plasma Biosci Res Ctr, Seoul 139701, South KoreaKi Hong Choi - Kwangwoon UniversityYoung June Hong - Kwangwoon Univ, Plasma Biosci Res Ctr, Seoul 139701, South KoreaVandana Miller - Drexel UniversityAlexander Fridman - Drexel UniversityEun Ha Choi - Kwangwoon University
- Publication Details
- Journal of physics. D, Applied physics, v 49(8), pp 84001-84009
- Publisher
- Iop Publishing Ltd
- Number of pages
- 9
- Grant note
- CURE NRF-2010-0027963; 2015R1C1A1A02036389 / National Research Foundation of Korea (NRF) - Korean government (MSIP) Kwangwoon University
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology; Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:000369480800004
- Scopus ID
- 2-s2.0-84957561834
- Other Identifier
- 991019168655004721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Physics, Applied