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Journal article
D-Amino Acids Inhibit Initial Bacterial Adhesion: Thermodynamic Evidence
Biotechnology and bioengineering, v 112(4), pp 696-704
Apr 2015
PMID: 25333717
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Bacterial biofilms are structured communities of cells enclosed in a self-produced hydrated polymeric matrix that can adhere to inert or living surfaces. D-Amino acids were previously identified as self-produced compounds that mediate biofilm disassembly by causing the release of the protein component of the polymeric matrix. However, whether exogenous D-amino acids could inhibit initial bacterial adhesion is still unknown. Here, the effect of the exogenous amino acid D-tyrosine on initial bacterial adhesion was determined by combined use of chemical analysis, force spectroscopic measurement, and theoretical predictions. The surface thermodynamic theory demonstrated that the total interaction energy increased with more D-tyrosine, and the contribution of Lewis acid-base interactions relative to the change in the total interaction energy was much greater than the overall nonspecific interactions. Finally, atomic force microscopy analysis implied that the hydrogen bond numbers and adhesion forces decreased with the increase in D-tyrosine concentrations. D-Tyrosine contributed to the repulsive nature of the cell and ultimately led to the inhibition of bacterial adhesion. This study provides a new way to regulate biofilm formation by manipulating the contents of D-amino acids in natural or engineered systems. Biotechnol. Bioeng. 2015;112: 696-704. (c) 2014 Wiley Periodicals, Inc.
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Details
- Title
- D-Amino Acids Inhibit Initial Bacterial Adhesion: Thermodynamic Evidence
- Creators
- Su-Fang Xing - Shandong UniversityXue-Fei Sun - Shandong UniversityAlicia A. Taylor - Univ Calif Riverside, Dept Chem & Environm Engn, Riverside, CA 92521 USASharon L. Walker - University of California, RiversideYi-Fu Wang - Shandong UniversityShu-Guang Wang - Shandong University
- Publication Details
- Biotechnology and bioengineering, v 112(4), pp 696-704
- Publisher
- Wiley
- Number of pages
- 9
- Grant note
- JQ201116 / Natural Science Foundation of Shandong Province 20120131120017 / Specialized Research Fund for the Doctoral Program of Higher Education; Specialized Research Fund for the Doctoral Program of Higher Education (SRFDP) 51208283; 51178254 / National Natural Science Foundation of China; National Natural Science Foundation of China (NSFC) T32 ES018827 / National Research Service Award Institutional Training Grant T32ES018827 / NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Environmental Health Sciences (NIEHS)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- College of Engineering
- Web of Science ID
- WOS:000350474000009
- Scopus ID
- 2-s2.0-84924057449
- Other Identifier
- 991021229996204721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biotechnology & Applied Microbiology