Journal article
DBI-1, a Novel Gene Related to the Notch Family, Modulates Mitogenic Response to Insulin-like Growth Factor 1
Experimental cell research, v 238(2), pp 359-370
01 Feb 1998
PMID: 9473344
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The insulin-like growth factor 1 (IGF-1) receptor has been found to transform fibroblast cells when overexpressed. The removal of 108 aa from the C-terminus of the IGF-1 receptor abolishes the transforming ability of the receptor without affecting its ability to induce cell growth. The availability of this mutant receptor provides a means to examine the changes in gene expression which take place during transformation, solely in response to an increased number of IGF-1 receptors. Using differential display, we have examined differences in gene expression between cells expressing a wild-type, transforming IGF-1 receptor and cells expressing a C-terminally truncated, nontransforming IGF-1 receptor. We have cloned a novel 6.3-kb cDNA transcript (DBI-1) which is expressed at much lower levels in cells containing the wild-type IGF-1 receptor. The predicted protein sequence of DBI-1 contains seven EGF-like repeats, which bear >90% sequence identity to the rat Notch 2 protein. The cDNA also contains a potential DEAD box in the C-terminal region. The DBI-1 message is detected at relatively high levels in cardiac tissue and at lower levels in lung, liver, and kidney. Antibodies generated to a unique region of the DBI-1 protein recognize a protein of 88 kDa, which is localized in the nucleus. Overexpression of DBI-1 in cells which contain the wild-type IGF-1 receptor diminishes the mitogenic response to IGF-1.
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Details
- Title
- DBI-1, a Novel Gene Related to the Notch Family, Modulates Mitogenic Response to Insulin-like Growth Factor 1
- Creators
- Henry B. Hoff - Allegheny CollegeMaria Tresini - Allegheny CollegeShu Li - Allegheny CollegeChristian Sell - Allegheny College
- Publication Details
- Experimental cell research, v 238(2), pp 359-370
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000072002800007
- Scopus ID
- 2-s2.0-0032004720
- Other Identifier
- 991019169671604721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Cell Biology
- Oncology