Journal article
DNA-based HIV vaccines do not induce generalized activation in mucosal tissue T cells
Human vaccines & immunotherapeutics, v 8(11), pp 1648-1653
01 Nov 2012
PMID: 23111167
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
HIV preferentially infects activated T cells, and activated mucosal CD4
+
T cells are the primary sites of viral replication. One potential explanation for increased HIV acquisition rates in the STEP study is that vaccination with adenoviral (Ad) vectors increased CD4
+
T cell activation levels at the site of infection, a concept that others and we continue to explore.
1
,
2
Whether vaccination with HIV vaccine platforms increases the activation state of CD4
+
T cells within peripheral tissues, such as the gastro-intestinal (GI) mucosa, is exceptionally important to determine as a vaccine safety measure, given the susceptibility of activated CD4
+
T cells to HIV infection.
In this study we examined whether vaccination with DNA plasmids and chemokine adjuvants alter the activation state of T cells within the GI mucosa, inguinal LN, and peripheral blood. T cell activation state was measured by expression of CD25, CD69, and HLA-DR over the course of the prime/boost study. DNA plasmid vaccination did not increase expression of any of these markers in the 3 tissues studied. Addition of the gut-homing chemokine TECK during DNA plasmid vaccination did not alter activation levels of CD4
+
T cells at any of these sites. These findings indicate that DNA vaccines do not elicit generalized mucosal T cell activation. Thus, DNA platforms may be especially suitable for HIV vaccine development, where bystander activation could promote increased HIV transmission.
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Details
- Title
- DNA-based HIV vaccines do not induce generalized activation in mucosal tissue T cells
- Creators
- Morgan A Reuter - Department of Microbiology; Perelman School of Medicine; University of Pennsylvania; Philadelphia, PA USASally Yuan - Department of Microbiology; Perelman School of Medicine; University of Pennsylvania; Philadelphia, PA USAPreston A Marx - Tulane National Primate Research Center; Covington, LA USAMichele A Kutzler - Department of Medicine; Drexel University; Philadelphia, PA USADavid B Weiner - Department of Pathology and Laboratory of Medicine; University of Pennsylvania; Philadelphia, PA USAMichael R Betts - Department of Microbiology; Perelman School of Medicine; University of Pennsylvania; Philadelphia, PA USA
- Publication Details
- Human vaccines & immunotherapeutics, v 8(11), pp 1648-1653
- Publisher
- Landes Bioscience
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Infectious Diseases (and HIV Medicine)
- Web of Science ID
- WOS:000311897900019
- Scopus ID
- 2-s2.0-84869814768
- Other Identifier
- 991014877922904721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biotechnology & Applied Microbiology
- Immunology