Journal article
DNA orientation during electrophoresis in a viscoelastic solution
Separations technology, v 4(1), pp 55-61
1994
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The objective of the present study was to examine the possibility of using a viscoelastic (free) solution to separate DNA molecules. The electrophoretic orientation of λ DNA in an uncross-linked polyacrylamide solution was measured by a fluorescence-detected linear dichroism of bound ethidium bromide. The λ DNA molecules oriented almost parallel to the applied electric field direction during electrophoresis in a relatively low concentration of the polyacrylamide solution for a moderate electric field strength of 90 V/cm, and the extent of orientation increased with the field strength. The analogy between the DNA molecule and a thin cylindrical body in the polyacrylamide solution suggests that when one perfects the separation device, very large DNA molecules can be separated using a viscoelastic solution.
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Details
- Title
- DNA orientation during electrophoresis in a viscoelastic solution
- Creators
- Dong-Wook Chee - Drexel UniversityYoung I. Cho - Drexel UniversityKeumnam Cho - Sungkyunkwan UniversityPonzy Lu - University of Pennsylvania
- Publication Details
- Separations technology, v 4(1), pp 55-61
- Publisher
- Elsevier Ireland Ltd
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:A1994MR83200006
- Scopus ID
- 2-s2.0-0028317709
- Other Identifier
- 991019174742504721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Chemistry, Analytical
- Engineering, Chemical