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DNA-synthesis regulation and correlation with inositol trisphosphate levels in cultured bovine retinal capillary pericytes
Journal article   Peer reviewed

DNA-synthesis regulation and correlation with inositol trisphosphate levels in cultured bovine retinal capillary pericytes

Weiye Li, Lei Tang, Qi Zhou, Mei Qin and Hu Tiansheng
Experimental eye research, v 49(4), pp 677-683
1989
DOI: https://doi.org/10.1016/S0014-4835(89)80062-4
PMID: 2806431
Featured in Collection :   UN Sustainable Development Goals @ Drexel

Abstract

aldose reductase inhibitor diabetic retinopathy DNA synthesis inositol trisphosphate pericyte
Inositol phosphate (IP), inositol bisphosphate (IP 2) and inositol trisphosphate (IP 3) in culturedbovine retinal capillary pericytes (BRCP) were quantitated by an ion-pair reverse-phase HPLC. BRCP were grown in media with standard (5 mM) or high (30 mM) glucose, and were either labeled with myo-[2- 3H]inositol (20 μCi ml −1) for 60 hr or with dual isotopes (20 μCi ml −1 myo-[2- 3H]inositol and 2 μCi ml −1 [ 14C]glycerol) for 8 hr. In parallel, BRCP in different glucose-media were incubated with 1 μCi ml −1 [ 3H]thymidime for 4 hr. High glucose significantly suppressed the accumulation of [ 3H]label in IP, IP 2 and IP 3, and specifically reduced the incorporation of [ 14C]glycerol into inositol phospholipids, but not that of neutral lipids and other types of phospholipids. The reduced IP 3 level correlated with the decrease in the incorporation of [ 3H]thymidine into DNA. Both the reduced IP 3 formation and DNA synthesis which were induced by high glucose were significantly reversed by adding either myo-inositol or Al1576, an aldose reductase inhibitor (ARI). However, the addition of neither myo-inositol nor. ARI stimulated IP 3 formation and/or DNA synthesis when BRCP were grown in the standard medium (5 mμ glucose). These findings indicate that myo-inositol metabolism and the polyol pathway affect inositol phospholipid-mediated pericyte division in vitro only under the highglucose condition. These data are compatible with the hypothesis that altered inositol phospholipid metabolism accounts for the loss of pericytes in diabetic retinopathy.

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Ophthalmology
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