Journal article
DPPH and oxygen free radicals as pro-oxidant of biomolecules
Toxicology in vitro, v 22(2)
01 Mar 2008
PMID: 17888621
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Numerous investigations exist about the alterations that oxygen free radicals can provoke on biomolecules; these modifications can be prevented and/or reversed by different antioxidants agents. On the other hand, 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), a stable nitrogen synthetic radical, is used to evaluate the antioxidant capacity of medicinal herbal products; however, the structural changes that this radical provoke on the herbal active principles are not clear yet. In this work, we compared the redox reactivity of oxygen free radicals and DPPH radical on phospholipids and protein thiol groups present in rat liver microsomes. Cu2+/ascorbate was used as generator system of oxygen free radical and as antioxidant, an extract of Buddleja globosa's leaves. Cu2+/ascorbate provoked microsomal lipid peroxidation, microsomal thiols oxidation and oxygen consumption; all of these phenomena were inhibited by B. globosa extract. On the other hand, DPPH was bleached in different extension by the herbal extract and phosphatidyl choline; beside, DPPH decreased microsomal thiols content, but this phenomenon were not prevented by the herbal extract. Furthermore, DPPH did not induce oxygen consumption and neither modified the oxygen consumption induced by Cu2+/ascorbate. Distinct redox mechanisms may explain the differences between the reactivity of DPPH and oxygen free radicals on biomolecules, which is discussed. (C) 2007 Elsevier Ltd. All rights reserved.
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Details
- Title
- DPPH and oxygen free radicals as pro-oxidant of biomolecules
- Creators
- Maria Eugenia Letelier - University of ChileAlfredo Molina-Berrios - University of ChileJuan Cortes-Troncoso - University of ChileJose Jara-Sandoval - University of ChileMarianne Holst - University of ChileKarina Palma - University of ChileMargarita Montoya - University of ChileDante Miranda - University of ChileVictor Gorizalez-Lira - University of Chile
- Publication Details
- Toxicology in vitro, v 22(2)
- Publisher
- Elsevier
- Number of pages
- 8
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems; Drexel University
- Web of Science ID
- WOS:000254694500001
- Scopus ID
- 2-s2.0-38749094435
- Other Identifier
- 991019415662304721
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- Web of Science research areas
- Toxicology