Journal article
Danazol alters mitochondria metabolism of fibrocystic breast Mcf10A cells
Breast (Edinburgh), v 35
01 Oct 2017
PMID: 28649033
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Fibrocystic Breast Disease (FBD) or Fibrocystic change (FC) affects about 60% of women at some time during their life. Although usually benign, it is often associated with pain and tenderness (mastalgia). The synthetic steroid danazol has been shown to be effective in reducing the pain associated with FBD, but the cellular and molecular mechanisms for its action have not been elucidated. We investigated the hypothesis that danazol acts by affecting energy metabolism. Effects of danazol on Mcf10A cells homeostasis, including mechanisms of oxidative phosphorylation, cytosolic calcium signaling and oxidative stress, were assessed by high-resolution respirometry and flow cytometry. In addition to fast physiological responses the associated genomic modulations were evaluated by Affimetrix microarray analysis. The alterations of mitochondria membrane potential and respiratory activity, downregulation of energy metabolism transcripts result in suppression of energy homeostasis and arrest of Mcf10A cells growth. The data obtained in this study impacts the recognition of direct control of mitochondria by cellular mechanisms associated with altered energy metabolism genes governing the breast tissue susceptibility and response to medication by danazol. (C) 2017 Elsevier Ltd. All rights reserved.
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Details
- Title
- Danazol alters mitochondria metabolism of fibrocystic breast Mcf10A cells
- Creators
- Zhazira Irgebay - Drexel UniversityBanu Yeszhan - Al-Farabi Kazakh National UniversityBhaswati Sen - Drexel UniversitySultan Tuleukhanov - Al-Farabi Kazakh National UniversityAri D. Brooks - University of PennsylvaniaRichard Sensenig - University of PennsylvaniaZulfiya Orynbayeva - Drexel University
- Publication Details
- Breast (Edinburgh), v 35
- Publisher
- Elsevier
- Number of pages
- 8
- Grant note
- Mary DeWitt Pettit grant Cornelius Beukenkamp endowment
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology; Surgery
- Web of Science ID
- WOS:000410705500010
- Scopus ID
- 2-s2.0-85021144939
- Other Identifier
- 991019168266804721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Obstetrics & Gynecology
- Oncology