Decorin Regulates the Aggrecan Network Integrity and Biomechanical Functions of Cartilage Extracellular Matrix

Biao Han; Qing Li; Chao Wang; Pavan Patel; Sheila M. Adams; Basak Doyran; Hadi T. Nia; Ramin Oftadeh; Siyuan Zhou; Christopher Y. Li; X. Sherry Liu; X. Lucas Lu; Motomi Enomoto-Iwamoto; Ling Qin; Robert L. Mauck; Renato V. Iozzo; David E. Birk; Lin Han

doi:10.1021/acsnano.9b04477

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Decorin Regulates the Aggrecan Network Integrity and Biomechanical Functions of Cartilage Extracellular Matrix

Journal article

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Decorin Regulates the Aggrecan Network Integrity and Biomechanical Functions of Cartilage Extracellular Matrix

Biao Han, Qing Li, Chao Wang, Pavan Patel, Sheila M. Adams, Basak Doyran, Hadi T. Nia, Ramin Oftadeh, Siyuan Zhou, Christopher Y. Li, …

ACS nano, v 13(10), pp 11320-11333

01 Oct 2019

DOI: https://doi.org/10.1021/acsnano.9b04477

PMID: 31550133

Featured in Collection : UN Sustainable Development Goals @ Drexel

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Abstract

Chemistry

Chemistry, Multidisciplinary

Chemistry, Physical

Materials Science, Multidisciplinary

Nanoscience & Nanotechnology

Science & Technology

Science & Technology - Other Topics

Materials Science

Physical Sciences

Technology

Joint biomechanical functions rely on the integrity of cartilage extracellular matrix. Understanding the molecular activities that govern cartilage matrix assembly is critical for developing effective cartilage regeneration strategies. This study elucidated the role of decorin, a small leucine-rich proteoglycan, in 20 the structure and biomechanical functions of cartilage. In decorin- null cartilage, we discovered a substantial reduction of aggrecan content, the major proteoglycan of cartilage matrix, and mild changes in collagen fibril nanostructure. This loss of aggrecan resulted in significantly impaired biomechanical properties of cartilage, including decreased modulus, elevated hydraulic permeability, and reduced energy dissipation capabilities. At the cellular level, we found that decorin functions to increase the retention of aggrecan in the neo-matrix of chondrocytes, rather than to directly influence the biosynthesis of aggrecan. At the molecular level, we demonstrated that decorin significantly increases the adhesion between aggrecan and aggrecan molecules and between aggrecan molecules and collagen II fibrils. We hypothesize that decorin plays a crucial structural role in mediating the matrix integrity and biomechanical functions of cartilage by providing physical linkages to increase the adhesion and assembly of aggrecan molecules at the nanoscale.

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Title: Decorin Regulates the Aggrecan Network Integrity and Biomechanical Functions of Cartilage Extracellular Matrix
Creators: Biao Han - Drexel University
Qing Li - Drexel University
Chao Wang - Drexel University
Pavan Patel - Drexel University
Sheila M. Adams - University of South Florida
Basak Doyran - Drexel University
Hadi T. Nia - Boston University
Ramin Oftadeh - Massachusetts Institute of Technology
Siyuan Zhou - Southwest University
Christopher Y. Li - Drexel University
X. Sherry Liu - Raymond and Ruth Perelman School of Medicine at the University of Pennsylvania
X. Lucas Lu - University of Delaware
Motomi Enomoto-Iwamoto - University of Maryland, Baltimore
Ling Qin - Raymond and Ruth Perelman School of Medicine at the University of Pennsylvania
Robert L. Mauck - Raymond and Ruth Perelman School of Medicine at the University of Pennsylvania
Renato V. Iozzo - Thomas Jefferson University
David E. Birk - University of South Florida
Lin Han - Drexel University
Publication Details: ACS nano, v 13(10), pp 11320-11333
Publisher: American Chemical Society; Washington, DC
Number of pages: 14
Grant note: CMMI-1662544 / National Science Foundation (NSF) R21AR066824 / NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Arthritis & Musculoskeletal & Skin Diseases (NIAMS) AR066824 / National Institutes of Health (NIH); United States Department of Health & Human Services; National Institutes of Health (NIH) - USA P30 AR069619 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
Resource Type: Journal article
Language: English
Academic Unit: School of Biomedical Engineering, Science, and Health Systems; Materials Science and Engineering
Web of Science ID: WOS:000492801600042
Scopus ID: 2-s2.0-85073005628
Other Identifier: 991019168506604721

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Collaboration types: Domestic collaboration; International collaboration
Web of Science research areas: Chemistry, Multidisciplinary; Chemistry, Physical; Materials Science, Multidisciplinary; Nanoscience & Nanotechnology

Decorin Regulates the Aggrecan Network Integrity and Biomechanical Functions of Cartilage Extracellular Matrix

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UN Sustainable Development Goals (SDGs)

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