Journal article
Defining pre-synaptic nicotinic receptors regulated by beta amyloid in mouse cortex and hippocampus with receptor null mutants
Journal of neurochemistry, v 109(5), pp 1452-1458
Jun 2009
PMID: 19457164
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Disruption of neuronal signaling by soluble beta-amyloid has been implicated in deficits in short-term recall in the early stages of Alzheimer's disease. One potential target for beta-amyloid is the synapse, with evidence for differential interaction with both pre- and post-synaptic elements. Our previous work revealed an agonist-like action of soluble beta-amyloid (pM to nM) on isolated pre-synaptic terminals to increase [Ca2+]i, with apparent involvement of pre-synaptic nicotinic receptors. To directly establish the role of nicotinic receptors in pre-synaptic Ca2+ regulation, we investigated the pre-synaptic action of beta-amyloid on terminals isolated from mice harboring either beta 2 or alpha 7 nicotinic receptor null mutants (knockouts). Average pre-synaptic responses to beta-amyloid in hippocampal terminals of alpha 7 knockout mice were unchanged, whereas responses in hippocampal terminals from beta 2 knockout mice were strongly attenuated. In contrast, pre-synaptic responses to soluble beta-amyloid were strongly attenuated in cortical terminals from alpha 7 knockout mice but were moderately attenuated in cortical terminals from beta 2 knockout mice. The latter responses, having distinct kinetics, were completely blocked by alpha-bungarotoxin. The use of receptor null mutants thus permitted direct demonstration of the involvement of specific nicotinic receptors in pre-synaptic Ca2+ regulation by soluble beta-amyloid, and also indicated differential neuromodulation by beta-amyloid of synapses in hippocampus and cortex.
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Details
- Title
- Defining pre-synaptic nicotinic receptors regulated by beta amyloid in mouse cortex and hippocampus with receptor null mutants
- Creators
- Tejal K. Mehta - Drexel UniversityJohn J. Dougherty - Drexel UniversityJianlin Wu - Drexel UniversityCatherine H. Choi - Drexel UniversityGhous M. Khan - Drexel UniversityRobert A. Nichols - Drexel University
- Publication Details
- Journal of neurochemistry, v 109(5), pp 1452-1458
- Publisher
- Wiley
- Number of pages
- 7
- Grant note
- State of Pennsylvania Tobacco Formula Funds AG21586 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA R01AG021586 / NATIONAL INSTITUTE ON AGING; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute on Aging (NIA)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Obstetrics and Gynecology
- Web of Science ID
- WOS:000265935200022
- Scopus ID
- 2-s2.0-65549084830
- Other Identifier
- 991022193494304721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Neurosciences