Journal article
Dendritic cells are required for optimal activation of natural killer functions following primary infection with herpes simplex virus type 1
Journal of virology, v 83(7), pp 3175-3186
Apr 2009
PMID: 19144708
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Natural killer (NK) cells play an important role in the optimal clearance of herpes simplex virus type 1 (HSV-1) infection in mice. Activated NK cells function via cytokine secretion or direct cytolysis of target cells; dendritic cells (DCs) are thought to make critical contributions in the activation of both of these functions. Yet, the magnitude and physiological relevance of DC-mediated NK cell activation in vivo is not completely understood. To examine the contribution of DC help in regulating NK cell functions after infection with HSV-1, we utilized a transgenic mouse model that allows the transient ablation of DCs. Using this approach, it was found that the gamma interferon (IFN-gamma) expression potential of NK cells is quantitatively and qualitatively impaired in the absence of DCs. With regard to priming of NK cytolytic functions, the ablation of DCs did not significantly affect cytotoxic protein expression by NK cells. An in vivo cytolytic assay did, however, reveal impairments in the magnitude of NK cell cytotoxicity. Overall, this study provides direct evidence that functional DCs are required for optimal IFN-gamma expression and cytolytic function by NK cells following infection with HSV-1.
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Details
- Title
- Dendritic cells are required for optimal activation of natural killer functions following primary infection with herpes simplex virus type 1
- Creators
- Sadik H Kassim - Department of Microbiology and Immunology, Louisiana State University Health Sciences Center, Shreveport, Louisiana 71130, USANaveen K RajasagiBarry W RitzStephen B PruettElizabeth M GardnerRobert ChervenakStephen R Jennings
- Publication Details
- Journal of virology, v 83(7), pp 3175-3186
- Publisher
- American Society for Microbiology (ASM); United States
- Grant note
- R01-AI-49428 / NIAID NIH HHS R01 AI049428 / NIAID NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000264046000035
- Scopus ID
- 2-s2.0-63149095564
- Other Identifier
- 991014877707804721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Virology