Journal article
Dense fibrillar collagen is a potent inducer of invadopodia via a specific signaling network
The Journal of cell biology, v 208(3), pp 331-350
02 Feb 2015
PMID: 25646088
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Cell interactions with the extracellular matrix (ECM) can regulate multiple cellular activities and the matrix itself in dynamic, bidirectional processes. One such process is local proteolytic modification of the ECM. Invadopodia of tumor cells are actin-rich proteolytic protrusions that locally degrade matrix molecules and mediate invasion. We report that a novel high-density fibrillar collagen (HDFC) matrix is a potent inducer of invadopodia, both in carcinoma cell lines and in primary human fibroblasts. In carcinoma cells, HDFC matrix induced formation of invadopodia via a specific integrin signaling pathway that did not require growth factors or even altered gene and protein expression. In contrast, phosphoproteomics identified major changes in a complex phosphosignaling network with kindlin2 serine phosphorylation as a key regulatory element. This kindlin2-dependent signal transduction network was required for efficient induction of invadopodia on dense fibrillar collagen and for local degradation of collagen. This novel phosphosignaling mechanism regulates cell surface invadopodia via kindlin2 for local proteolytic remodeling of the ECM.
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Details
- Title
- Dense fibrillar collagen is a potent inducer of invadopodia via a specific signaling network
- Creators
- Vira V Artym - Georgetown University Medical CenterStephen Swatkoski - National Institutes of HealthKazue Matsumoto - National Institutes of HealthCatherine B Campbell - National Institutes of HealthRyan J Petrie - National Institutes of HealthEmilios K Dimitriadis - National Institutes of HealthXin Li - Georgetown University Medical CenterSusette C Mueller - Georgetown University Medical CenterThomas H Bugge - National Institutes of HealthMarjan Gucek - National Institutes of HealthKenneth M Yamada - National Institutes of Health
- Publication Details
- The Journal of cell biology, v 208(3), pp 331-350
- Grant note
- K99 CA129205 / NCI NIH HHS DE 000719 / NIDCR NIH HHS K99CA129205 / NCI NIH HHS ZIA DE000719 / Intramural NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Web of Science ID
- WOS:000348972000007
- Scopus ID
- 2-s2.0-84961290125
- Other Identifier
- 991020099170404721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Cell Biology