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Journal article
Depletion of kinesin-12, amyosin-IIB-interacting protein, promotes migration of cortical astrocytes
Journal of cell science, v 129(12), pp 2438-2447
15 Jun 2016
PMID: 27170353
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Kinesin-12 (also named Kif15) participates in important events during neuronal development, such as cell division of neuronal precursors, migration of young neurons and establishment of axons and dendritic arbors, by regulating microtubule organization. Little is known about the molecular mechanisms behind the functions of kinesin-12, and even less is known about its roles in other cell types of the nervous system. Here, we show that kinesin-12 depletion from cultured rat cortical astrocytes decreases cell proliferation but increases migration. Co-immunoprecipitation, GST pulldown and small interfering RNA (siRNA) experiments indicated that kinesin-12 directly interacts with myosin-IIB through their tail domains. Immunofluorescence analyses indicated that kinesin-12 and myosin-IIB colocalize in the lamellar region of astrocytes, and fluorescence resonance energy transfer analyses revealed an interaction between the two. The phosphorylation at Thr1142 of kinesin-12 was vital for their interaction. Loss of their interaction through expression of a phosphorylation mutant of kinesin-12 promoted astrocyte migration. We suggest that kinesin-12 and myosin-IIB can form a hetero-oligomer that generates force to integrate microtubules and actin filaments in certain regions of cells, and in the case of astrocytes, that this interaction can modulate their migration.
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Details
- Title
- Depletion of kinesin-12, amyosin-IIB-interacting protein, promotes migration of cortical astrocytes
- Creators
- Jie Feng - Nantong UniversityZunlu Hu - Nantong UniversityHaijiao Chen - Nantong UniversityJuan Hua - Nantong UniversityRonghua Wu - Nantong UniversityZhangji Dong - Nantong UniversityLiang Qiang - Drexel UniversityYan Liu - Nantong Univ, Coinnovat Ctr Neuroregenerat, Jiangsu Key Lab Neuroregenerat, Nantong 226001, Jiangsu, Peoples R ChinaPeter W. Baas - Drexel UniversityMei Liu - Nantong University
- Publication Details
- Journal of cell science, v 129(12), pp 2438-2447
- Publisher
- COMPANY OF BIOLOGISTS LTD
- Number of pages
- 10
- Grant note
- 11KJA180004 / Jiangsu Provincial Department of Education R01 NS28785 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA Jiangsu Provincial Department of Education [Priority Academic Program Development (PAPD)] R01NS028785 / NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS) KYZZ14_0354; CXLX13_868 / Scientific innovation project 31171007; 31371078 / National Natural Science Foundation of China; National Natural Science Foundation of China (NSFC) BK20150404 / Natural Science Foundation of Jiangsu Province
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000379498500015
- Scopus ID
- 2-s2.0-84976503031
- Other Identifier
- 991019168834504721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Cell Biology