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Journal article
Designing broad-spectrum anti-HIV-1 gRNAs to target patient-derived variants
Scientific reports, v 7(1), pp 14413-7
31 Oct 2017
PMID: 29089503
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Clustered regularly interspaced short palindromic repeats (CRISPR) CRISPR-associated protein 9 (Cas9), including specific guide RNAs (gRNAs), can excise integrated human immunodeficiency virus type 1 (HIV-1) provirus from host chromosomes. To date, anti-HIV-1 gRNAs have been designed to account for off-target activity, however, they seldom account for genetic variation in the HIV-1 genome within and between patients, which will be crucial for therapeutic application of this technology. This analysis tests the ability of published anti-HIV-1 gRNAs to cleave publicly available patient-derived HIV-1 sequences to inform gRNA design and provides basic computational tools to researchers in the field.
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Details
- Title
- Designing broad-spectrum anti-HIV-1 gRNAs to target patient-derived variants
- Creators
- Will Dampier - Drexel UniversityNeil T. Sullivan - Drexel UniversityCheng-Han Chung - Drexel UniversityJoshua Chang Mell - Drexel UniversityMichael R. Nonnemacher - Drexel UniversityBrian Wigdahl - Drexel University
- Publication Details
- Scientific reports, v 7(1), pp 14413-7
- Publisher
- Springer Nature
- Number of pages
- 7
- Grant note
- R01MH110360 / NATIONAL INSTITUTE OF MENTAL HEALTH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Mental Health (NIMH) R01 MH110360 / Public Health Service, National Institutes of Health, through grants from the National Institute of Mental Health (NIMH) T32 MH079785 / Ruth L. Kirschstein National Research Service Award; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA P30 MH092177 / NIMH Comprehensive NeuroAIDS Center (CNAC)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000414231000014
- Scopus ID
- 2-s2.0-85032616602
- Other Identifier
- 991019168326604721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biotechnology & Applied Microbiology