Journal article
Desmin cytoskeletal modifications after a bout of eccentric exercise in the rat
American journal of physiology. Regulatory, integrative and comparative physiology, v 283(4), pp R958-R963
01 Oct 2002
PMID: 12228066
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Desmin content and immunohistochemical appearance were measured in tibialis anterior muscles of rats subjected to a single bout of 30 eccentric contractions (ECs). Ankle torque was measured before EC and at various recovery times, after which immunohistochemical and immunoblot analyses were performed. Torque decreased by ∼50% immediately after EC and fully recovered 168 h later ( P < 0.001). Loss of desmin staining was maximal 12 h after EC and recovered by 72 h. Immunoblots unexpectedly demonstrated a significant increase in the desmin-to-actin ratio by 72 h after EC ( P < 0.01) and was still increasing after 168 h ( P < 0.0001). These data demonstrate a relatively rapid qualitative loss of desmin immunostaining immediately after a single EC bout but a tremendous quantitative increase in desmin content 72–168 h later. This dynamic restructuring of the muscle's intermediate filament system may be involved in the mechanism of EC-induced muscle injury and may provide a structural explanation for the protective effects observed in muscle after a single EC bout.
Metrics
Details
- Title
- Desmin cytoskeletal modifications after a bout of eccentric exercise in the rat
- Creators
- Ilona A. Barash - San Francisco VA Medical CenterDavid Peters - San Francisco VA Medical CenterJan Fridén - Sahlgrenska University HospitalGordon J. Lutz - San Francisco VA Medical CenterRichard L. Lieber - San Francisco VA Medical Center
- Publication Details
- American journal of physiology. Regulatory, integrative and comparative physiology, v 283(4), pp R958-R963
- Publisher
- American Physiological Society (APS)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000177960000020
- Scopus ID
- 2-s2.0-0036784561
- Other Identifier
- 991021463689304721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Physiology