Journal article
Detailing organelle division and segregation in Plasmodium falciparum
The Journal of cell biology, v 223(12)
02 Dec 2024
PMID: 39485315
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The malaria-causing parasite, P. falciparum, replicates through schizogony, a tightly orchestrated process where numerous daughter parasites are formed simultaneously. Proper division and segregation of one-per-cell organelles, like the mitochondrion and apicoplast, are essential, yet remain poorly understood. We developed a new reporter parasite line that allows visualization of the mitochondrion in blood and mosquito stages. Using high-resolution 3D imaging, we found that the mitochondrion orients in a cartwheel structure, prior to stepwise, non-geometric division during last-stage schizogony. Analysis of focused ion beam scanning electron microscopy data confirmed these mitochondrial division stages. Furthermore, these data allowed us to elucidate apicoplast division steps, highlighted its close association with the mitochondrion, and showed putative roles of the centriolar plaques in apicoplast segregation. These observations form the foundation for a new detailed mechanistic model of mitochondrial and apicoplast division and segregation during P. falciparum schizogony and pave the way for future studies into the proteins and protein complexes involved in organelle division and segregation.
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Details
- Title
- Detailing organelle division and segregation in Plasmodium falciparum
- Creators
- Julie M J Verhoef - Radboud University NijmegenCas Boshoven - Radboud University NijmegenFelix Evers - Radboud University NijmegenLaura J Akkerman - Radboud University NijmegenBarend C A Gijsbrechts - Radboud University NijmegenMarga van de Vegte-Bolmer - Radboud University NijmegenGeert-Jan van Gemert - Radboud University Medical CenterAkhil B Vaidya - Drexel UniversityTaco W A Kooij - Radboud University Nijmegen
- Publication Details
- The Journal of cell biology, v 223(12)
- Publisher
- ROCKEFELLER UNIV PRESS; NEW YORK
- Number of pages
- 31
- Grant note
- R01 AI028398 / NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:001346021200001
- Scopus ID
- 2-s2.0-85208291882
- Other Identifier
- 991021955314704721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Cell Biology