Journal article
Detect of Escherichia coli O157:H7 in ground beef samples using piezoelectric excited millimeter-sized cantilever (PEMC) sensors
Biosensors & bioelectronics, v 22(7), pp 1296-1302
15 Feb 2007
PMID: 16831542
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Piezoelectric-excited millimeter-sized cantilever (PEMC) sensors consisting of a piezoelectric and a borosilicate glass layer with a sensing area of 4mm2 were fabricated. An antibody specific to Escherichia coli (anti-E. coli) O157:H7 was immobilized on PEMC sensors, and exposed to samples containing E. coli O157:H7 (EC) prepared in various matrices: (1) broth, broth plus raw ground beef, and broth plus sterile ground beef without inoculation of E. coli O157:H7 served as controls, (2) 100mL of broth inoculated with 25 EC cells, (3) 100mL of broth containing 25g of raw ground beef and (4) 100mL of broth with 25g of sterile ground beef inoculated with 25 EC cells. The total resonant frequency change obtained for the broth plus EC samples were 16±2Hz (n=2), 30Hz (n=1), and 54±2Hz (n=2) corresponding to 2, 4, and 6h growth at 37°C, respectively. The response to the broth plus 25g of sterile ground beef plus EC cells were 21±2Hz (n=2), 37Hz (n=1), and 70±2Hz (n=2) corresponding to 2, 4, and 6h, respectively. In all cases, the three different control samples yielded a frequency change of 0±2Hz (n=6). The E. coli O157:H7 concentration in each broth and beef samples was determined by both plating and by pathogen modeling program. The results indicate that the PEMC sensor detects E. coli O157:H7 reliably at 50–100cells/mL with a 3mL sample.
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Details
- Title
- Detect of Escherichia coli O157:H7 in ground beef samples using piezoelectric excited millimeter-sized cantilever (PEMC) sensors
- Creators
- Gossett A Campbell - Department of Chemical and Biological Engineering, Drexel University, Philadelphia, PA 19104, United StatesJoe Uknalis - US Department of Agriculture, Agricultural Research Service, NAA, Eastern Regional Research Center, 600 E. Mermaid Lane, Wyndmoor, PA 19038, United StatesShu-I Tu - US Department of Agriculture, Agricultural Research Service, NAA, Eastern Regional Research Center, 600 E. Mermaid Lane, Wyndmoor, PA 19038, United StatesRaj Mutharasan - Department of Chemical and Biological Engineering, Drexel University, Philadelphia, PA 19104, United States
- Publication Details
- Biosensors & bioelectronics, v 22(7), pp 1296-1302
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemical and Biological Engineering
- Web of Science ID
- WOS:000244385400015
- Scopus ID
- 2-s2.0-33846355681
- Other Identifier
- 991014877967104721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biophysics
- Biotechnology & Applied Microbiology
- Chemistry, Analytical
- Electrochemistry
- Nanoscience & Nanotechnology