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Detection of Heme-Distortions in Ferri- and Ferrocyto-Chrome C by Resonance Raman Scattering
Journal article   Peer reviewed

Detection of Heme-Distortions in Ferri- and Ferrocyto-Chrome C by Resonance Raman Scattering

U. Kubitschek, W. Dreybrodt and R. Schweitzer-stenner
Spectroscopy letters, v 19(6), pp 681-690
01 Jul 1986

Abstract

cytochrome c distortions of the heme group Resonance Raman scattering
We have measured the depolarization ratio and polarized intensities of the oxidation marker A 1g -Raman line at 1363 cm −1 and 1375 cm −1 in ferri- and ferrocytochrome c respectively. From these data we derive symmetry classified distortions of the heme group from its ideal D 4h -symmetry. In ferrocytochrome c these distortions are independent of the pH-value of the solution, indicating that the heme environment is stable with respect to pH. In ferricytochrome c we observe pH-dependent changes of the distortions, indicating the well known conformational change with pK = 9.05, where the methionine 80 ligand is replaced by lysine-79. The data indicate that in this conformation due to opening of the heme crevice the heme group has a higher effective symmetry.

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Spectroscopy
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