Journal article
Detection of Heme-Distortions in Ferri- and Ferrocyto-Chrome C by Resonance Raman Scattering
Spectroscopy letters, v 19(6), pp 681-690
01 Jul 1986
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Abstract
We have measured the depolarization ratio and polarized intensities of the oxidation marker A
1g
-Raman line at 1363 cm
−1
and 1375 cm
−1
in ferri- and ferrocytochrome c respectively. From these data we derive symmetry classified distortions of the heme group from its ideal D
4h
-symmetry. In ferrocytochrome c these distortions are independent of the pH-value of the solution, indicating that the heme environment is stable with respect to pH. In ferricytochrome c we observe pH-dependent changes of the distortions, indicating the well known conformational change with pK = 9.05, where the methionine 80 ligand is replaced by lysine-79. The data indicate that in this conformation due to opening of the heme crevice the heme group has a higher effective symmetry.
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Details
- Title
- Detection of Heme-Distortions in Ferri- and Ferrocyto-Chrome C by Resonance Raman Scattering
- Creators
- U. Kubitschek - University of BremenW. Dreybrodt - University of BremenR. Schweitzer-stenner - University of Bremen
- Publication Details
- Spectroscopy letters, v 19(6), pp 681-690
- Publisher
- Taylor & Francis Group
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemistry
- Web of Science ID
- WOS:A1986C998400009
- Scopus ID
- 2-s2.0-0001442161
- Other Identifier
- 991019196684204721
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- Web of Science research areas
- Spectroscopy