Journal article
Detection of airborne Bacillus anthracis spores by an integrated system of an air sampler and a cantilever immunosensor
Sensors and actuators. B, Chemical, v 127(2), pp 376-382
2007
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
A near real-time, sensitive and reliable method of detecting airborne
Bacillus anthracis spores by air sampling (wet mode) and then exposing the collected sample to an antibody-functionalized piezoelectric-excited millimeter-sized cantilever (PEMC) sensor is presented. Using a commercial air sampler, a 10
min air sample at 267
L/min captured the airborne particulates containing
Bacillus anthracis (BA) spores and concentrated them into 5
mL of phosphate buffered saline (PBS). This sample was then injected into a flow cell containing an antibody-functionalized PEMC sensor. The resonant frequency of the PEMC sensor at 925.1
kHz decreased exponentially as the BA spores attached to the sensor surface producing a positive response well beyond the noise level in 2
min and reached a steady state value in 20
min. In liquid phase, the sensor response correlated well (
R
2
=
0.99) with spore concentration and was shown to follow: [Response in Hz]
=
(0.0637)
×
(spore concentration in #/mL). Our results show that detection of 38
BA spores/L of air is achievable in near real-time with an estimated lower limit of detection of ∼5
spores/L of air in the configuration tested.
Metrics
Details
- Title
- Detection of airborne Bacillus anthracis spores by an integrated system of an air sampler and a cantilever immunosensor
- Creators
- Gossett A CampbellDavid deLesdernierRaj Mutharasan
- Publication Details
- Sensors and actuators. B, Chemical, v 127(2), pp 376-382
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemical and Biological Engineering
- Web of Science ID
- WOS:000251228600011
- Scopus ID
- 2-s2.0-35348966338
- Other Identifier
- 991014878548604721
UN Sustainable Development Goals (SDGs)
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Chemistry, Analytical
- Electrochemistry
- Instruments & Instrumentation