Journal article
Detection of pathogen Escherichia coli O157:H7 AT 70 cells/mL using antibody-immobilized biconical tapered fiber sensors
Biosensors & bioelectronics, v 21(6), pp 871-880
2005
PMID: 16257655
Abstract
Optical fibers (core diameter 8
μm, cladding diameter 125
μm) was tapered to a waist diameter in the range of 8–12
μm, and then a monoclonal antibody to the pathogen,
Escherichia coli O157:H7 was covalently bonded to the surface of the tapered region. Using 470
nm light, the taper was exposed to various concentrations (7
×
10
7, 7
×
10
5, 7
×
10
3, and 70
cells/mL) of the pathogen, and the sensor showed changes in transmitted light as the antigen attached to the antibody on the taper surface. The response was equal and opposite when the pathogen was released from the surface using a low pH buffer. The magnitude of the change was inversely proportional to the concentration of the pathogen. The sensor showed good sensitivity at as low a concentration as 70
cells/mL. The antibody-immobilized taper sensor was also exposed to a mixture of the pathogen and a non-pathogenic variant (JM101) at 0%, 50% and 70% by concentration. The sensor showed good selectivity to the pathogenic antigen. A first order attachment kinetic model is proposed to quantify the rate of attachment of pathogen to the sensor surface. The kinetic rate constant (
k) of
E. coli O157:H7 to the fiber was found to vary in the range of (2.5–6.1)
×
10
−9
min
−1
(cells/mL)
−1.
Metrics
Details
- Title
- Detection of pathogen Escherichia coli O157:H7 AT 70 cells/mL using antibody-immobilized biconical tapered fiber sensors
- Creators
- Kishan Rijal - Department of Chemical Engineering, Drexel University, Philadelphia, PA 19104, USAAngela Leung - Department of Chemical Engineering, Drexel University, Philadelphia, PA 19104, USAP. Mohana Shankar - Department of Electrical and Computer Engineering, Drexel University, Philadelphia, PA 19104, USARaj Mutharasan - Department of Chemical Engineering, Drexel University, Philadelphia, PA 19104, USA
- Publication Details
- Biosensors & bioelectronics, v 21(6), pp 871-880
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Electrical and Computer Engineering; Chemical and Biological Engineering
- Web of Science ID
- WOS:000233346300006
- Scopus ID
- 2-s2.0-27444442945
- Other Identifier
- 991014878413304721
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biophysics
- Biotechnology & Applied Microbiology
- Chemistry, Analytical
- Electrochemistry
- Nanoscience & Nanotechnology