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Detection of prohibited substances in equestrian sport through direct injection of equine serum using micellar LC
Journal article   Peer reviewed

Detection of prohibited substances in equestrian sport through direct injection of equine serum using micellar LC

David P Thomas and Joe P Foley
Bioanalysis, v 2(2)
Feb 2010
PMID: 21083306

Abstract

Substance Abuse Detection - methods Injections Limit of Detection Animals Micelles Horses Linear Models Anti-Inflammatory Agents, Non-Steroidal - isolation & purification Chromatography, Liquid - methods Anti-Inflammatory Agents, Non-Steroidal - blood Doping in Sports
Detection of prohibited substances in equestrian sports typically involves time-consuming and tedious sample-preparation methods. Micellar LC (MLC) allows for direct injection of equine serum to detect prohibited NSAIDs. The method was linear over the range of standards examined, with recoveries of 94.2-95.1% for phenylbutazone (12-18 µg/ml), and 83.9-88.7% and 87.9-105.0% for diclofenac and flunixin, respectively (0.1-1.2 µg/ml). The limit of detection was 0.1 µg/ml for all compounds and the limit of quantitation was 0.2 µg/ml for phenylbutazone and 0.3 µg/ml for diclofenac and flunixin. The MLC method of direct equine serum injection was shown to be valid and simple for the quantitation of banned NSAIDs in equine serum.

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Web of Science research areas
Biochemical Research Methods
Chemistry, Analytical
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