Journal article
Determination of mitochondrial metabolic phenotype through investigation of the intrinsic inhibition of succinate dehydrogenase
Analytical biochemistry, v 552, pp 30-37
01 Jul 2018
PMID: 29042133
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Many diseases are accompanied by systemic or organ metabolic abnormalities. Therefore, investigation of the roles of mitochondrial dysfunction in the pathogenesis of major diseases requires a methodology that reflects the characteristics of mitochondrial metabolism particular for the organ of origin. We provide evidence that for brain and heart mitochondria the intrinsic inhibition of succinate dehydrogenase (SDH) is a key mechanism for attenuation of mitochondrial respiration and energy production in response to the organ's energy needs. This mechanism also serves to minimize the production of reactive oxygen species when the organ is at rest. Changes in the organ's workloads are accompanied by changes in metabolites that are used by mitochondria as substrates and for modification of energy production at the SDH level. Measurement of the respiratory activity of mitochondria with various substrates and substrate mixtures and use of bovine serum albumin as an SDH inhibitor will be useful for evaluation of metabolic phenotype at the mitochondrial level.
•Intrinsic inhibition of SDH is an important mechanism for attenuation of mitochondrial respiration to the organ's energy demands.•The properties of intrinsic inhibition of SDH are organ and species specific.•Energy production and generation of ROS by mitochondria are controlled metabolically at the level of SDH.
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Details
- Title
- Determination of mitochondrial metabolic phenotype through investigation of the intrinsic inhibition of succinate dehydrogenase
- Creators
- Alexander Panov - Institute of Molecular Biology and BiophysicsZulfiya Orynbayeva - Drexel University
- Publication Details
- Analytical biochemistry, v 552, pp 30-37
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Surgery
- Web of Science ID
- WOS:000439741200005
- Scopus ID
- 2-s2.0-85049120136
- Other Identifier
- 991019167411004721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemical Research Methods
- Biochemistry & Molecular Biology
- Chemistry, Analytical